Cilj studije: Istražiti izražaj gangliozida GM3 i njegovih sfingolipidnih preteča u mišiću na štakorskom modelu šećerne bolesti tipa 2. Metode i materijali: U istraživanju su korišteni muški Sprague-Dawley štakori. Šećerna bolest inducirana je kombinacijom streptozotocina (35 mg/kg) i prehrane s visokim udjelom masti - 58%. Štakorima je redovito mjerena tjelesna masa i koncentracija glukoze u plazmi. Dva tjedan nakon induciranja šećerne bolesti tipa 2, štakori su žrtvovani, a sadržaj GM3 ispitan imunofluorescentnom mikroskopijom i tankoslojnom kromatografijom visokog razlučivanja. Sadržaj glikosfinoglipidnih preteča gangliozida GM3 (ceramid, glukozilceramid i laktozilceramid) u tkivu mišića je analiziran metodom tankoslojne kromatografije visokog razlučivanja. Rezultati: Tkivo mišića ispitivane DMT2 skupine štakora imalo je statistički značajno povišene razine gangliozida GM3 u odnosnu na kontrolnu C-DMT2 skupinu (P<0,001). Razine glikosfingolipidnih preteča gangliozida GM3 (ceramid, glukozilceramid, laktozilceramid) su takoĎer bile značajno povišene (P<0,05). Zaključak: Statistički značajno povišene razine glangliozida GM3 u ispitivanoj DMT2 skupini štakora u odnosu na ispitivanu C-DMT2 skupinu, kao i njegovih glikosfingolipidnih preteča, u skladu su s nalazima dosadašnjih studija. Navedeno upućuje na ključnu ulogu gangliozida GM3 u razvoju inzulinske rezistencije u poprečno-prugastoj muskulaturi. Štakorski model šećerne bolesti tipa 2 inducirane streptozotocinom i prehranom s visokim udjelom masti, pokazao se kao adekvatan za istraživanje utjecaja glikosfingolipida na inzulinsku rezistenciju.

Objectives: To investigate the expression of ganglioside GM3 and its glycosphingolipid precursors in muscle in rat model of type 2 diabetes. Methods: In the research were used male Sprague-Dawley rats. Diabetes type 2 was induced using combination of streptozotocin (35 mg/kg) and diet with high share of fat - 58%. Rats weight and plasma glucose were regularly measured. Two weeks after diabetes type 2 induction, rats were sacrificed, and the content of GM3 was analyzed using imunofluorescence microscopy and high performance thin layer chromatography (HPTLC). Intramuscular expression of sphingolipid precursors of GM3 (ceramide, glucosylceramide, lactosylceramide) wa analyzed using high performance thin layer chromatography (HPTLC). Results: Statistically significant intramuscular increase of ganglioside GM3 levels was observed in the tested DMT2 group, compared to the control C-DMT2 group (P<0.001). Levels of glycosphingolipid precursors of ganglioside GM3 (ceramide, glucosilceramide, lactosylceramide) were also significantly increased (P<0.05). Conclusion: Significantly increased levels of ganglioside GM3 in the investigated DMT2 group, compared to the control C-DMT2 group confirms previous research. Ganglioside GM3 has crucial role in striated muscle in development of insulin resistance. The rat model for diabetes type 2 induced by streptozotocine and high-fat diet, proved to be adequate for research of glycosphingolipid impact in development of insulin resistance.