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THE EXPRESSION OF GANGLIOSIDE GM3 AND ITS SPHINGOLIPID PRECURSORS IN MUSCLE IN RAT MODELS OF TYPE 2 DIABETES
Hrvoje Vučemilović (2014)
University of Split
School of Medicine
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Vučemilović, H. (2014). IZRAŽAJ GANGLIOZIDA GM3 I NJEGOVIH SFINGOLIPIDNIH PRETEČA U MIŠIĆU NA ŠTAKORSKOM MODELU ŠEĆERNE BOLESTI TIPA 2 (Master's thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:240704

Vučemilović, Hrvoje. "IZRAŽAJ GANGLIOZIDA GM3 I NJEGOVIH SFINGOLIPIDNIH PRETEČA U MIŠIĆU NA ŠTAKORSKOM MODELU ŠEĆERNE BOLESTI TIPA 2." Master's thesis, University of Split, School of Medicine, 2014. https://urn.nsk.hr/urn:nbn:hr:171:240704

Vučemilović, Hrvoje. "IZRAŽAJ GANGLIOZIDA GM3 I NJEGOVIH SFINGOLIPIDNIH PRETEČA U MIŠIĆU NA ŠTAKORSKOM MODELU ŠEĆERNE BOLESTI TIPA 2." Master's thesis, University of Split, School of Medicine, 2014. https://urn.nsk.hr/urn:nbn:hr:171:240704

Vučemilović, H. (2014). 'IZRAŽAJ GANGLIOZIDA GM3 I NJEGOVIH SFINGOLIPIDNIH PRETEČA U MIŠIĆU NA ŠTAKORSKOM MODELU ŠEĆERNE BOLESTI TIPA 2', Master's thesis, University of Split, School of Medicine, accessed 18 July 2019, https://urn.nsk.hr/urn:nbn:hr:171:240704

Vučemilović H. IZRAŽAJ GANGLIOZIDA GM3 I NJEGOVIH SFINGOLIPIDNIH PRETEČA U MIŠIĆU NA ŠTAKORSKOM MODELU ŠEĆERNE BOLESTI TIPA 2 [Master's thesis]. Split: University of Split, School of Medicine; 2014 [cited 2019 July 18] Available at: https://urn.nsk.hr/urn:nbn:hr:171:240704

H. Vučemilović, "IZRAŽAJ GANGLIOZIDA GM3 I NJEGOVIH SFINGOLIPIDNIH PRETEČA U MIŠIĆU NA ŠTAKORSKOM MODELU ŠEĆERNE BOLESTI TIPA 2", Master's thesis, University of Split, School of Medicine, Split, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:171:240704

Metadata
TitleIZRAŽAJ GANGLIOZIDA GM3 I NJEGOVIH SFINGOLIPIDNIH PRETEČA U MIŠIĆU NA ŠTAKORSKOM MODELU ŠEĆERNE BOLESTI TIPA 2
Title (english)THE EXPRESSION OF GANGLIOSIDE GM3 AND ITS SPHINGOLIPID PRECURSORS IN MUSCLE IN RAT MODELS OF TYPE 2 DIABETES
AuthorHrvoje Vučemilović
MentorTina Tičinović Kurir (mentor)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2014, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Internal Medicine
Abstract
Cilj studije: Istražiti izražaj gangliozida GM3 i njegovih sfingolipidnih preteča u mišiću na štakorskom modelu šećerne bolesti tipa 2. Metode i materijali: U istraživanju su korišteni muški Sprague-Dawley štakori. Šećerna bolest inducirana je kombinacijom streptozotocina (35 mg/kg) i prehrane s visokim udjelom masti - 58%. Štakorima je redovito mjerena tjelesna masa i koncentracija glukoze u plazmi. Dva tjedan nakon induciranja šećerne bolesti tipa 2, štakori su žrtvovani, a sadržaj GM3 ispitan imunofluorescentnom mikroskopijom i tankoslojnom kromatografijom visokog razlučivanja. Sadržaj glikosfinoglipidnih preteča gangliozida GM3 (ceramid, glukozilceramid i laktozilceramid) u tkivu mišića je analiziran metodom tankoslojne kromatografije visokog razlučivanja. Rezultati: Tkivo mišića ispitivane DMT2 skupine štakora imalo je statistički značajno povišene razine gangliozida GM3 u odnosnu na kontrolnu C-DMT2 skupinu (P<0,001). Razine glikosfingolipidnih preteča gangliozida GM3 (ceramid, glukozilceramid, laktozilceramid) su takoĎer bile značajno povišene (P<0,05). Zaključak: Statistički značajno povišene razine glangliozida GM3 u ispitivanoj DMT2 skupini štakora u odnosu na ispitivanu C-DMT2 skupinu, kao i njegovih glikosfingolipidnih preteča, u skladu su s nalazima dosadašnjih studija. Navedeno upućuje na ključnu ulogu gangliozida GM3 u razvoju inzulinske rezistencije u poprečno-prugastoj muskulaturi. Štakorski model šećerne bolesti tipa 2 inducirane streptozotocinom i prehranom s visokim udjelom masti, pokazao se kao adekvatan za istraživanje utjecaja glikosfingolipida na inzulinsku rezistenciju.
Abstract (english)
Objectives: To investigate the expression of ganglioside GM3 and its glycosphingolipid precursors in muscle in rat model of type 2 diabetes. Methods: In the research were used male Sprague-Dawley rats. Diabetes type 2 was induced using combination of streptozotocin (35 mg/kg) and diet with high share of fat - 58%. Rats weight and plasma glucose were regularly measured. Two weeks after diabetes type 2 induction, rats were sacrificed, and the content of GM3 was analyzed using imunofluorescence microscopy and high performance thin layer chromatography (HPTLC). Intramuscular expression of sphingolipid precursors of GM3 (ceramide, glucosylceramide, lactosylceramide) wa analyzed using high performance thin layer chromatography (HPTLC). Results: Statistically significant intramuscular increase of ganglioside GM3 levels was observed in the tested DMT2 group, compared to the control C-DMT2 group (P<0.001). Levels of glycosphingolipid precursors of ganglioside GM3 (ceramide, glucosilceramide, lactosylceramide) were also significantly increased (P<0.05). Conclusion: Significantly increased levels of ganglioside GM3 in the investigated DMT2 group, compared to the control C-DMT2 group confirms previous research. Ganglioside GM3 has crucial role in striated muscle in development of insulin resistance. The rat model for diabetes type 2 induced by streptozotocine and high-fat diet, proved to be adequate for research of glycosphingolipid impact in development of insulin resistance.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:240704
Study programmeTitle: Medicina
Study programme type: sveučilišni
Study level: integrirani preddiplomski i diplomski
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsRestricted access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
Created on2016-03-09 08:02:17