Title Biokompatibilnost materijala za pečaćenje Title (english) Biocompatibility of dental sealants Author Anita Buterin Mentor Lidia Gavić (mentor)Committee member Antonija Tadin (predsjednik povjerenstva)Committee member Livia Cigić (član povjerenstva)Committee member Ivan Galić (član povjerenstva)Granter University of Split Defense date and country 2019, Croatia Scientific / art field, BIOMEDICINE AND HEALTHCARE Abstract Cilj: Glavni cilj ovog istraživanja bio je istražiti biokompatibilnost materijala za pečaćenje, odnosno ispitati njihov utjecaj na stanice bukalne sluznice.
Materijali i metode: U ovom istraživanju sudjelovalo je 45 ispitanika, 27 djevojčica i 18 dječaka; u dobi od 7 do 16 godina (srednja dob 12,09 ± 2,20), koji su pristupili pregledu i pečaćenju zubi na Odjelu za dječju dentalnu medicinu Stomatološke poliklinike Split te Zavodu za dječju stomatologiju Stomatološkog fakulteta u Zagrebu.
Bris bukalne sluznice uzet je neposredno prije postavljanja materijala za pečaćenje te 7, 30 i 90 dana nakon postavljanja pečata. Pacijenti ili njihovi staratelji su ispunili i upitnik vezan za demografske podatke (dob, spol), te podatke o načinu prehrane, zdravstvenom statusu, korištenju lijekova te nedavnoj izloženosti RTG zrakama.
Rezultati: Uočena je statistički značajna razlika u broju stanica s kondenziranim kromatinom za Helioseal F® između vremena prije postavljanja materijala za pečaćenje te 90 dana nakon postavljanja (P=0,025). Za ostala dva korištena materijala za pečaćenje nije uočena značajna razlika. Izloženost RTG zračenju te konzumacija lijekova, mesa, slatkiša, gaziranih napitaka i čaja utječe značajno na nastanak kondenziranog kromatina, dok spol utječe na nastanak binukleusa.
Zaključak: Niti jedan ispitivani materijal nije pokazao statistički značajni porast patoloških promjena jezgre tijekom kraćeg vremenskog perioda. Nakon 90 dana, samo je Helioseal F® uzrokovao statistički značajni porast stanica s kondenziranim kromatinom. Iako je riječ o materijalima koji se već koriste u kliničkoj praksi, od velike je važnosti ovakvim in vivo kliničkim studijama provjeriti njegovu biokompatibilnost.
Abstract (english) Objectives: The main purpose of this study was to investigate the effects of dental sealants on buccal cells to assess their biocompatibility.
Materials and methods: This study was conducted on 45 patients (27 girls and 18 boys), 7 to 16 years of age (age mean 12,09 ± 2,20) and carried out in the Pediatric dentistry department in Dental clinic in Split and Pediatric dentistry department on Faculty of dentistry in Zagreb.
Buccal swabs (samples) were collected previous to, and also 7, 30 and 90 days after fissure sealant placement. Patients or their parents/legal guardians filled in the questionnaire regarding demographic data (age, gender), dietary habits, health status, medication usage, and recent X-ray exposure.
Results: Statistically significant difference in the number of buccal cells with condensed chromatin between T0 (time before fissure sealant placement) and T4 (90 days after fissure sealant placement) period for Helioseal F® (P=0,025) was noticed. The other two analyzed materials did not show any significant difference. Factors such as recent X-ray exposure, medication usage, meat, candy, aerated beverages, and tea affect the development of nuclear anomalies such as condensed chromatin, whereas age induces the development of binucleated cells.
Conclusion: Neither of the three tested dental sealants showed a statistically significant rise in the number of nuclear anomalies during a short period. During 90 days period, only Helioseal F® caused a statistically significant increase in the number of cells with condensed chromatin. Although these sealants are already in use, it is of high importance that their biocompatibility is constantly checked, especially in vivo clinical studies.
Keywords
Zubni karijes
Materijali za pečaćenje
Ispitivanje materijala
Testovi mikronukleusa
Keywords (english)
Dental Caries
Pit and Fissure Sealants
Materials Testing
Micronucleus Tests
Language croatian URN:NBN urn:nbn:hr:171:276939 Study programme Title: Dental Medicine Type of resource Text File origin Born digital Access conditions Access restricted to students and staff of home institution Terms of use Created on 2021-06-24 09:58:06
