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master's thesis
KORELACIJA EKSPRESIJE SATB1 I Ki-67 S KROMOSOMSKIM ABERACIJAMA U BOLESNIKA S AKUTNOM MIJELOIČNOM LEUKEMIJOM I MIJELODISPLASTIČNIM SINDROMOM
2021. urn:nbn:hr:171:422246
Dilber, Ana
University of Split
School of Medicine

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Dilber, A. (2021). KORELACIJA EKSPRESIJE SATB1 I Ki-67 S KROMOSOMSKIM ABERACIJAMA U BOLESNIKA S AKUTNOM MIJELOIČNOM LEUKEMIJOM I MIJELODISPLASTIČNIM SINDROMOM (Master's thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:422246

Dilber, Ana. "KORELACIJA EKSPRESIJE SATB1 I Ki-67 S KROMOSOMSKIM ABERACIJAMA U BOLESNIKA S AKUTNOM MIJELOIČNOM LEUKEMIJOM I MIJELODISPLASTIČNIM SINDROMOM." Master's thesis, University of Split, School of Medicine, 2021. https://urn.nsk.hr/urn:nbn:hr:171:422246

Dilber, Ana. "KORELACIJA EKSPRESIJE SATB1 I Ki-67 S KROMOSOMSKIM ABERACIJAMA U BOLESNIKA S AKUTNOM MIJELOIČNOM LEUKEMIJOM I MIJELODISPLASTIČNIM SINDROMOM." Master's thesis, University of Split, School of Medicine, 2021. https://urn.nsk.hr/urn:nbn:hr:171:422246

Dilber, A. (2021). 'KORELACIJA EKSPRESIJE SATB1 I Ki-67 S KROMOSOMSKIM ABERACIJAMA U BOLESNIKA S AKUTNOM MIJELOIČNOM LEUKEMIJOM I MIJELODISPLASTIČNIM SINDROMOM', Master's thesis, University of Split, School of Medicine, accessed 19 May 2022, https://urn.nsk.hr/urn:nbn:hr:171:422246

Dilber A. KORELACIJA EKSPRESIJE SATB1 I Ki-67 S KROMOSOMSKIM ABERACIJAMA U BOLESNIKA S AKUTNOM MIJELOIČNOM LEUKEMIJOM I MIJELODISPLASTIČNIM SINDROMOM [Master's thesis]. Split: University of Split, School of Medicine; 2021 [cited 2022 May 19] Available at: https://urn.nsk.hr/urn:nbn:hr:171:422246

A. Dilber, "KORELACIJA EKSPRESIJE SATB1 I Ki-67 S KROMOSOMSKIM ABERACIJAMA U BOLESNIKA S AKUTNOM MIJELOIČNOM LEUKEMIJOM I MIJELODISPLASTIČNIM SINDROMOM", Master's thesis, University of Split, School of Medicine, Split, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:171:422246

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TitleKORELACIJA EKSPRESIJE SATB1 I Ki-67 S KROMOSOMSKIM ABERACIJAMA U BOLESNIKA S AKUTNOM MIJELOIČNOM LEUKEMIJOM I MIJELODISPLASTIČNIM SINDROMOM
Title (english)CORRELATION OF SATB1 AND Ki-67 EXPRESSION WITH CHROMOSOMAL ABERRATIONS IN PATIENTS WITH ACUTE MYELOID LEUKEMIA AND MYELODYSPLASTIC SYNDROME
AuthorAna Dilber
MentorBernarda Lozić (mentor)
Committee memberJoško Markić (predsjednik povjerenstva)
Committee memberSlavica Dajak (član povjerenstva)
Committee memberIvana Pavlinac Dodig (član povjerenstva)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2021, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Internal Medicine
Abstract
Cilj istraživanja: Ciljevi istraživanja bili su odrediti ekspresiju proteina SATB1 i Ki-67 iz arhiviranih uzoraka fiksiranih stanica suspenzija koštane srži bolesnika s AML-om i MDS-om i utvrditi moguće korelacije njihove ekspresije s kontrolnom skupinom, kliničko-patološkim podatcima i citogenetičko-prognostičkim skupinama. Ispitanici i metode: U ovo retrospektivno istraživanje bilo je uključeno 30 bolesnika iznad 18 godina, s dijagnozom AML-a i MDS-a u ispitivanoj skupini. Kontrolna skupina ispitanika uključivala je 23 bolesnika koji su podvrgnuti biopsiji koštane srži u dijagnostičke svrhe, a u kojih je isključena maligna bolest hematopoetskog sustava. Svi bolesnici obrađeni su pri Klinici za dječje bolesti KBC-a Split, u razdoblju od lipnja 2018. do svibnja 2021. Prikazani su podatci o citogenetičkom nalazu, spolu, dobi, broju leukocita, eritrocita, trombocita, koncentraciji hemoglobina i laktat dehidrogenaze u vrijeme inicijalne dijagnoze. Analiza uzoraka stanica koštane srži izvršila se imunofluorescencijom, metodom dvostrukog bojanja. Rezultati: U ovom istraživanju pronađene su slične ekspresije SATB1 (P=0,101) i Ki-67 (P=0,149) u ispitivanoj i kontrolnoj skupini. U studiji smo pokazali niže ekspresije SATB1 ovisno o citogenetičkom nalazu u usporedbi s kontrolnom skupinom, a 91% bolesnika iz skupine AML/MDS s kompleksnim kariotipom ima negativnu ekpresiju na SATB1 (P=0,045). Srednja vrijednost dobi u ispitivanoj skupini bolesnika s negativnom ekspresijom na SATB1 bila je 68,53 godina, dok je srednja vrijednost dobi u bolesnika s pozitivnom ekspresijom bila 55 godina (P=0,082). Srednje vrijednosti ekspresije Ki-67 u kontrolnoj (25,96%) i ispitivanoj skupini (24,77%) bile su gotovo podjednake. Zaključci: Kromatin remodelirajući protein, SATB1, ima nedostatnu ekspresiju u specifičnom biološkom entitetu mijeloidnih poremećaja ovisno o citogenetičkom nalazu, a najniža ekspresija se pokazala u bolesnika muškog spola, starije životne dobi i s kompleksnim kariotipom te može biti važan čimbenik u leukemogenezi.
Abstract (english)
Objectives: The aim of the study was to determine expression of SATB1 and Ki-67 in archived bone marrow aspirate samples of patients with AML and MDS and the correlation of their expression with control group, clinical-pathological information and cytogenetic risk categories. Materials and methods: This retrospective study included 30 patients above age of 18 with the diagnosis of AML and MDS. Control group consisted of 23 patients, without hematologic malignancy, who underwent bone marrow biopsy for diagnostic purposes. All patients were processed in Cytogenetics laboratory of the Clinic for Children's Diseases of the University Hospital of Split from June 2018. until May 2021. Data included cytogenetic findings, gender, age, blood cell count, hemoglobin concentration and lactate dehydrogenase levels at the diagnosis. Bone marrow specimen were analyzed using double immunofluorescence staining. Results: This study showed similar expression of SATB1 (P=0,101) and Ki-67 (P=0,149) in examined and control group of patients. There was significantly lower SATB1 expression depending on cytogenetic findings (P=0,045), in comparison to control group, and 91% of patients with complex karyotype had negative SATB1 expression. Mean age of patients in examined group with negative SATB1 expression was 68,53 years, while the mean age of those with positive SATB1 expression was 55 years (P = 0,082). Mean values of Ki-67 were almost equal, 25,96% and 24,77% in control and examined group, respectively. Conclusions: Chromatin-remodeling protein, SATB1, expression was inadequate in specific biologic entity of myeloid disorders depending on cytogenetic findings, and the lowest expression was found in males of older age, and complex karyotype, which suggest that it can play an important role in leukemogenesis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:422246
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-07-13 13:29:43