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master's thesis
Procjena genotoksičnosti i citotoksičnosti dentalnih implantata na gingivnim epitelnim stanicama
2020. urn:nbn:hr:171:238174
Radović, Mirna
University of Split
School of Medicine
Endodoncy and restorative dentistry

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Radović, M. (2020). Procjena genotoksičnosti i citotoksičnosti dentalnih implantata na gingivnim epitelnim stanicama (Master's thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:238174

Radović, Mirna. "Procjena genotoksičnosti i citotoksičnosti dentalnih implantata na gingivnim epitelnim stanicama." Master's thesis, University of Split, School of Medicine, 2020. https://urn.nsk.hr/urn:nbn:hr:171:238174

Radović, Mirna. "Procjena genotoksičnosti i citotoksičnosti dentalnih implantata na gingivnim epitelnim stanicama." Master's thesis, University of Split, School of Medicine, 2020. https://urn.nsk.hr/urn:nbn:hr:171:238174

Radović, M. (2020). 'Procjena genotoksičnosti i citotoksičnosti dentalnih implantata na gingivnim epitelnim stanicama', Master's thesis, University of Split, School of Medicine, accessed 18 November 2024, https://urn.nsk.hr/urn:nbn:hr:171:238174

Radović M. Procjena genotoksičnosti i citotoksičnosti dentalnih implantata na gingivnim epitelnim stanicama [Master's thesis]. Split: University of Split, School of Medicine; 2020 [cited 2024 November 18] Available at: https://urn.nsk.hr/urn:nbn:hr:171:238174

M. Radović, "Procjena genotoksičnosti i citotoksičnosti dentalnih implantata na gingivnim epitelnim stanicama", Master's thesis, University of Split, School of Medicine, Split, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:171:238174

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Metadata
TitleProcjena genotoksičnosti i citotoksičnosti dentalnih implantata na gingivnim epitelnim stanicama
Title (english)Genotoxicity assessment of dental implants in gingival epithelial cells
AuthorMirna Radović
MentorAntonija Tadin (mentor)
Committee memberIvan Kovačić (predsjednik povjerenstva)
Committee memberLidia Gavić (član povjerenstva)
Committee memberMarina Ognjenović (član povjerenstva)
GranterUniversity of Split
School of Medicine
(Endodoncy and restorative dentistry)
Split
Defense date and country2020, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Dental Medicine
Oral Surgery
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Dental Medicine
Periodontology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Dental Medicine
Prosthetic Dentistry
Abstract
Cilj istraživanja: Iako se titanijum (Ti) temeljeni dentalni implantati smatraju bioinertnima, potvrđeno je kao su podložni koroziji i trošenju. Cilj ove in vivo provedene studije bio je procijeniti genotoksičan potencijal dvije vrste dentalni implantata u gingivnim epitelnim stanicama. Materijali i metode: U 78 ispitanika uzeti su uzorci oljuštenih gingivnih stanica tri puta. Prije postavljanja dentalnih implantata (T0), 90 dana nakon implantacije - neposredno prije postavljanja gingiva formera (T1) te 21 dan od postavljanja gingiva formera (T2). DNK oštećenje je procjenjivano mikronukleus testom. Rezultati: Dobiveni rezultati pokazuju kako nakon postavljanja obje vrste ispitivanih dentalnih implantata (Ankylos i Dentium) dolazi do značajnog povećanja broja binuklearnih stanica u odnosu na početne vrijednosti (T0 naspram T1; p = 0,011 i p ≤ 0,001). Također u odnosu na početne vrijednosti, nakon postavljanja gingiva formera (T0 naspram T2) kod ispitanika dolazi do značajnog povećanja broja stanica s mikronukleusom (p = 0,007 i p = 0,022) i binuklearnih stanica (p = 0,006 i p ≤ 0,001). Zaključak: Na osnovu dobivenih rezultata može se zaključiti kako titanijum temeljeni dentalni implantati ne izazivaju citotoksične i genotoksične učinke u gingivnim stanicama nakon postavljanja. Uočeni blagi učinci ne mogu se označiti kao biološki relevantni.
Abstract (english)
Objective: Although titanium (Ti) based implants are considered bioinert, it has been found that they can undergo corrosion and wear. The aim of this in vivo study was to evaluate genotoxic potential from two different implants in gingival epithelial cells. Material and methods: Exfoliated gingival cells were taken from 78 participants three times. Before implant insertion (T0), 90 days following dental implant insertion immediately before gingiva former placement (T1), and 21 days following gingiva former placement (T2). DNA damages were analyzed using the micronucleus test. Results: Obtained average cytogenetic damage values in individuals after implementation of both tested dental implants, Ankylos (Dentsply Implants Manufacturing GmbH, Mannheim, Germany) and Dentium (Dentium Co., Seoul, Korea), showed a significant increase only in the number of binuclei (T0 vs T1; p =0.011 and p ≤ 0.001). Until, frequency of cells with micronuclei (p = 0.007 and p = 0.022) and binuclei (p= 0.006 and p ≤ 0.001) were significantly higher 21 days following gingiva former placement (T0 vs T2). Conclusion: Based on the results, can be concluded that there is no Ti based implants dependent cytotoxic or genotoxic effect in gingival epithelial cells. The biological relevance of the observed slightly increased cytogenetic damages cannot be assessed.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:238174
Study programmeTitle: Dental Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica dentalne medicine (doktor/doktorica dentalne medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-09-06 13:04:59