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master's thesis
Izražaj RNazeH2A u mišićno-invazivnom urotelnom karcinomu mokraćnog mjehura
2014. urn:nbn:hr:171:892659
Jelović, Matej
University of Split
School of Medicine

Cite this document

Jelović, M. (2014). Izražaj RNazeH2A u mišićno-invazivnom urotelnom karcinomu mokraćnog mjehura (Master's thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:892659

Jelović, Matej. "Izražaj RNazeH2A u mišićno-invazivnom urotelnom karcinomu mokraćnog mjehura." Master's thesis, University of Split, School of Medicine, 2014. https://urn.nsk.hr/urn:nbn:hr:171:892659

Jelović, Matej. "Izražaj RNazeH2A u mišićno-invazivnom urotelnom karcinomu mokraćnog mjehura." Master's thesis, University of Split, School of Medicine, 2014. https://urn.nsk.hr/urn:nbn:hr:171:892659

Jelović, M. (2014). 'Izražaj RNazeH2A u mišićno-invazivnom urotelnom karcinomu mokraćnog mjehura', Master's thesis, University of Split, School of Medicine, accessed 26 January 2025, https://urn.nsk.hr/urn:nbn:hr:171:892659

Jelović M. Izražaj RNazeH2A u mišićno-invazivnom urotelnom karcinomu mokraćnog mjehura [Master's thesis]. Split: University of Split, School of Medicine; 2014 [cited 2025 January 26] Available at: https://urn.nsk.hr/urn:nbn:hr:171:892659

M. Jelović, "Izražaj RNazeH2A u mišićno-invazivnom urotelnom karcinomu mokraćnog mjehura", Master's thesis, University of Split, School of Medicine, Split, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:171:892659

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Metadata
TitleIzražaj RNazeH2A u mišićno-invazivnom urotelnom karcinomu mokraćnog mjehura
Title (english)Expression of RNaseH2A in muscle-invasive urothelial bladder carcinoma
AuthorMatej Jelović
MentorVedrana Čikeš Čulić (mentor)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2014, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Clinical Biochemistry
Abstract
Cilj istraživanja: Odrediti imunohistokemijski izražaj enzima RNazeH2A na parafinskim rezovima bioptičkih uzoraka pacijenata s urotelnim tumorima mokraćnog mjehura, te usporediti izražaj RNazeH2A u mišićno-neinvazivnim urotelnim tumorima s izražajem u mišićno-invazivnim urotelnim karcinomima mokraćnog mjehura. Materijali i metode: Istraživanje je provedeno na 20 bioptičkih uzoraka tumora mokraćnog mjehura uklopljenih u parafin, pacijenata koji su liječeni u Klinici za urologiju Kliničkog bolničkog centra Split. Uvidom u medicinsku dokumentaciju izdvojeno je 10 pacijenata s neinvazivnim urotelnim tumorom i 10 pacijenata s mišićno-invazivnim urotelnim karcinomom mokraćnog mjehura. Od svakog je pacijenta iz arhive Kliničkog odjela za patologiju, Kliničkog zavoda za patologiju sudsku medicinu i citologiju Kliničkog bolničkog centra Split, izdvojen po jedan parafinski blok s uzorkom tkiva. Uzorci tkiva ispitivani su na prisutnost te intezitet imunohistokemijskog izražaja enzima RNazeH2A primjenom metode imunohistokemije. Imunohistokemijski izražaj RNazeH2A je smatran pozitivnim ako su jezgre tumorskih stanica bile smeđe obojene, a intenzitet imunohistokemijskog izražaja RNazeH2A u tumorskim stanicama je opisan kao slabo pozitivan (+), umjereno pozitivan (++) i jako pozitivan (+++). Imunohistokemijski izražaj neinvazivnih tumora uspoređivan je s imunohistokemijskim izražajem mišićno-invazivnog urotelnog karcinoma mokraćnog mjehura. Rezultati: Dokazan je izražaj u svim uzorcima u obje skupine. U skupini mišićno-neinvazivnih urotelnih tumora mokraćnog mjehura 5 uzoraka (50 %) pokazalo je umjeren izražaj RNazeH2A (++), a 5 (50 %) jak izražaj (+++). U skupini mišićno-invazivog urotelnog karcinoma mokraćnog mjehura 1 uzorak (10 %) pokazao je slab izražaj (+), 2 uzorka (20 %) umjeren (++), a 7 uzoraka (70 %) pokazalo je jak izražaj. Zaključak: Usporedbom rezultata potvrdili smo hipotezu da je izražaj RNazeH2A jači što je tumor agresivniji. Ovaj rezultat ide u prilog studijama koje dokazuju pojačan izražaj enzima u stanicama karcinoma drugih tkiva i organa (22, 23). Određivanje izražaja RNazeH2A u urotelnim tumorima mokraćnog mjehura imunohistokemijskim metodama potvrđuje važnost ovog enzima u patogenezi, otkrivanju, praćenju tumora i razvijanja potencijalne ciljane terapije te otvara mnoga pitanja na koja bi trebale odgovoriti buduće studije na većem broju pacijenata.
Abstract (english)
Objectives: To determine the immunohistochemical expression of enzyme RNaseH2A on paraffin sections of biopsy samples of patients with urothelial bladder tumors, and to compare the expression of RNaseH2A in noninvasive urothelial bladder tumors with expression in muscle-invasive urothelial bladder carcinoma. Materials and Methods: The study included 20 biopsy samples of bladder tumors embedded in paraffin from the patients who were treated in the Department of Urology, University Hospital Split. After reviewing patient's medical records 10 patients with non-invasive urothelial bladder tumors and 10 with muscle-invasive urothelial bladder carcinoma were selected. One paraffin block of tissue sample of each patient was taken from the archives of the Clinical Department of Pathology, Clinical Institute of Pathology, Forensic Medicine and Cytology, University Hospital Split. Tissue samples were examined for the presence and intensity of immunohistochemical expression of enzyme RNazeH2A by immunohistochemical method. Immunohistochemical expression RNaseH2A was considered positive if the nucleus of tumor cells were stained brown, and the intensity of immunohistochemical expression RNaseH2A in tumor cells has been described as weakly positive (+), moderately positive (+ +) and strongly positive (+ + +). Immunohistochemical expression of non-invasive bladder tumors was then compared with the immunohistochemical expression of muscle-invasive urothelial bladder carcinoma. Results: The expression was detected in all samples in both groups. In the group of muscle-noninvasive urothelial bladder tumors 5 samples (50 %) showed moderate expression RNaseH2A (+) and 5 (50 %) strong expression (+ + +). In the group of muscle-invasive urothelial bladder carcinoma 1 sample (10 %) showed weak expression (+), 2 samples (20 %) moderate (+ +), and 7 samples (70 %) showed strong expression.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:892659
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
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Created on2016-03-14 07:47:23