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master's thesis
Antiproliferativno djelovanje izolata crnog i bijelog češnjaka
2022. urn:nbn:hr:171:354611
Prkić, Petra
University of Split
School of Medicine

Cite this document

Prkić, P. (2022). Antiproliferativno djelovanje izolata crnog i bijelog češnjaka (Master's thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:354611

Prkić, Petra. "Antiproliferativno djelovanje izolata crnog i bijelog češnjaka." Master's thesis, University of Split, School of Medicine, 2022. https://urn.nsk.hr/urn:nbn:hr:171:354611

Prkić, Petra. "Antiproliferativno djelovanje izolata crnog i bijelog češnjaka." Master's thesis, University of Split, School of Medicine, 2022. https://urn.nsk.hr/urn:nbn:hr:171:354611

Prkić, P. (2022). 'Antiproliferativno djelovanje izolata crnog i bijelog češnjaka', Master's thesis, University of Split, School of Medicine, accessed 13 December 2024, https://urn.nsk.hr/urn:nbn:hr:171:354611

Prkić P. Antiproliferativno djelovanje izolata crnog i bijelog češnjaka [Master's thesis]. Split: University of Split, School of Medicine; 2022 [cited 2024 December 13] Available at: https://urn.nsk.hr/urn:nbn:hr:171:354611

P. Prkić, "Antiproliferativno djelovanje izolata crnog i bijelog češnjaka", Master's thesis, University of Split, School of Medicine, Split, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:171:354611

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Metadata
TitleAntiproliferativno djelovanje izolata crnog i bijelog češnjaka
Title (english)Antiproliferative effect of black and fresh garlic isolates
AuthorPetra Prkić
MentorVedrana Čikeš Čulić (mentor)
Committee memberAnita Markotić (predsjednik povjerenstva)
Committee memberDiana Gujinović (član povjerenstva)
Committee memberVedrana Čikeš Čulić (član povjerenstva)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2022-10-14, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Medical Biochemistry
Abstract
Ciljevi: Cilj ovog istraživanja je ispitati citotoksično djelovanje uzoraka bijelog i crnog češnjaka (Allium sativum L.) dobivenih mikrovalnom ekstrakcijom na stanične linije humanog adenokarcinoma dojke MDA-MB-231 i humanog karcinoma mokraćnog mjehura T24. Pretpostavka je da će se broj karcinomskih stanica, uspoređujući s kontrolom, smanjiti nakon izlaganja biljnim uzorcima. Materijali i metode: Stanice su tretirane s uzorcima A. sativum L. dobivenim mikrovalnom ekstrakcijom (liofilizirani ostaci i mikrovalni ekstrakti bijelog i crnog češnjaka) u koncentraciji od 1 μg/mL, 5 μg/mL, 10 μg/mL, 50 μg/mL i 100 μg/mL. Citotoksična aktivnost odredila se MTT testom nakon 4, 24, 48 i 72 sata inkubacije. Spektrofotometrom je dobivena apsorbancija na 570 nm koja je korištena za određivanje djelotvornosti uzoraka češnjaka. Rezultati: Rezultati su prikazani grafički u odnosu koncentracije uzorka, vremena inkubacije i postotka metabolički aktivnih stanica. Kod stanične linije MDA-MB-231, liofilizirani ostaci bijelog i crnog češnjaka pokazuju neznatan citotoksični učinak. Mikrovalni ekstrakti bijelog i crnog češnjaka pokazuju učinak ovisan o koncentraciji i vremenu izloženosti. Duljom inkubacijom ostvaruje se veće smanjenje broja metabolički aktivnih stanica, a najizraženiji učinak je pri koncentracijama od 50 i 100 μg/mL. Kod stanične linije T24, liofilizirani ostaci bijelog i crnog češnjaka pokazuju neznatan citotoksični učinak. Mikrovalni ekstrakti bijelog i crnog češnjaka pokazuju učinak u svim ispitivanim koncentracijama, ali najizraženiji je pri 50 i 100 μg/mL. Značajna citotoksičnost postiže se nakon inkubacije od 72 sata. Zaključci: In vitro izlaganje staničnih linija humanih karcinoma dojke (MDA-MB-231) i mokraćnog mjehura (T24) uzorcima bijelog i crnog češnjaka uzrokuje smanjeno preživljavanje stanica. Citotoksični učinak ovisan je o koncentraciji i vremenu inkubacije. Učinkovitost je potvrđena za hlapljive spojeve sadržane u mikrovalnim ekstraktima, ali ne i za liofilizirane ostatke koji sadrže nehlapljive spojeve.
Abstract (english)
Objectives: The aims of this diploma thesis were to investigate the cytotoxic effects of black and white garlic isolates from microwave extraction on breast cancer cell line MDA-MB-231 and bladder cancer cell line T24. Materials and methods: The cells were treated with samples of A. sativum L. obtained by microwave extraction (lyophilized residues and microwave extracts of white and black garlic) at concentrations of 1 μg/mL, 5 μg/mL, 10 μg/mL, 50 μg/mL and 100 μg/mL. ml. Cytotoxic activity was determined by the MTT test after 4, 24, 48 and 72 hours of incubation. Absorbance at 570 nm was measured with a spectrophotometer, which was used to determine the effectiveness of garlic samples. Results: The results are presented graphically in relation to sample concentration, incubation time and percentage of metabolically active cells. In the MDA-MB-231 cell line, lyophilized residues of white and black garlic show a slight cytotoxic effect. Microwave extracts of white and black garlic show an effect dependent on concentration and exposure time. Longer incubation results in a greater reduction in the number of metabolically active cells, and the most pronounced effect is at concentrations of 50 and 100 μg/mL. In the T24 cell line, lyophilized residues of white and black garlic show a slight cytotoxic effect. Microwave extracts of white and black garlic show an effect in all tested concentrations, but it is most pronounced at 50 and 100 μg/mL. Significant cytotoxicity is achieved after 72 hours of incubation. Conclusions: In vitro exposure of human breast (MDA-MB-231) and bladder (T24) carcinoma cell lines to samples of white and black garlic causes decreased cell survival. The cytotoxic effect depends on the concentration and incubation time. Efficacy has been confirmed for volatile compounds contained in microwave extracts, but not for lyophilized residues containing non-volatile compounds.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:354611
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-10-17 11:11:25