| Abstract | Cilj: Ciljevi istraživanja bili su usporediti antimikrobnu učinkovitost biokeramičkih materijala za punjenje korijenskih kanala, te ispitati njihovu antibakterijsku učinkovitost na planktonske stanice i mlade biofilmove E. faecalis.
Materijali i metode: U prvom dijelu istraživanja pretražene su tri elektroničke baze podataka: MEDLINE preko OvidSP platforme, Embase preko OvidSP platforme i Web of Science. Istraživanja su uključena neovisno o dizajnu istraživanja, vrsti publikacije i jeziku. Dva autora su zasebno ocijenila kvalitetu izvještavanja.
U drugom dijelu istraživanja bakterijska suspenzija je postavljena na površinu svježe pripremljenih materijala te materijala koji su stvrdnjavali jedan ili tri dana u vertikalno postavljenim mikrotitarskim pločama. Nakon inkubacije 2, 5, 20 i 60 minuta u svaku jažicu mikrotitarske ploče dodan je triptaza soja bujon. Nakon serijskog razrjeđivanja, izbrojane su kolonije.
U trećem dijelu istraživanja bakterijski biofilmovi su uzgojeni na filter papirnatim diskovima postavljenima na ploče s agrom te su, nakon inkubacije, diskovi prekriveni s četiri materijala za punjenje korijenskih kanala. Nakon 60 minuta, materijali za punjenje su uklonjeni i diskovi su prenešeni u epruvete s fosfatom puferiranom fiziološkom otopinom. Nakon serijskog razrjeđivanja, izbrojane su kolonije.
Rezultati: Prvi dio istraživanja obuhvatio je 37 istraživanja koja su ispitivala antimikrobu učinkovitost biokeramičkih materijala za punjenje korijenskih kanala. Nije bilo moguće napraviti usporedbu rezultata istraživanja i dati jasan zaključak i usporedbu djelovanja jer su istraživači koristili različite izvore i starosne skupine mikroorganizama, različita vremena stvrdnjavanja materijala i kontakta između mikroorganizama i materijala i različite antimikrobne testove.
U drugom dijelu istraživanja su biokeramički materijali pokazali značajno bolju učinkovitost u odnosu na kontrolnu skupinu i materijal temeljen na umjetnoj smoli (p< 0.05). TotalFill BC Sealer (Brasseler SAD) je pokazao najbolju učinkovitost, usporedivu s BioRoot RCS-om (Septodont)(p> 0.05), a slijedi ih MTA Fillapex (Angelus) (p< 0.05). Svježe pripremljeni materijali su imali sličnu učinkovitost kao materijali koji su stvrdnjavali tri dana (p> 0.05), ali bolju od materijala koji su stvrdnjavali jedan dan (p< 0.05). Svi materijali su pokazali najbolju učinkovitost nakon 20 minuta kontakta s bakterijama, neovisno jesu bili svježe pripremljeni ili stvrdnjavali jedan ili tri dana.
U trećem dijelu istraživanja nije bilo značajne razlike između TotalFill BC Sealera (Brasseler SAD) i AH Plusa (Dentsply) (p=0.386). Oba materijala su pokazala bolju antibakterijsku učinkovitost od BioRoot RCS-a (Septodont) i MTA Fillapexa (Angelus) (p<0.001).
Zaključak: Budući da su istraživanja uključena u sustavni pregledni rad visoko heterogena, nije moguće donijeti zaključak o tome koji materijal ima najbolje djelovanje niti preporuke za kliničku praksu jer su istraživanja provođena na različite načine, što čini metaanalizu neizvedivom. Potrebno je usuglasiti metodološki pristup, jasno definirati podjelu materijala i provesti istraživanja na ljudima kako bi se moglo donijeti preporuke za kliničku praksu.
U istraživanju učinka biokeramičkih materijala na planktonske stanice E. faecalis, biokeramički materijali su pokazali bolju učinkovitost u usporedbi s konvencionalno korištenim materijalom temeljenim na umjetnoj smoli. Materijali su pokazali najbolju učinkovitost nakon 20 minuta kontakta s bakterijskom suspenzijom, neovisno o tome da li je materijal bio svježe pripremljen ili je stvrdnjavao jedan ili tri dana.
U istraživanju učinka biokeramičkih materijala na mlade biofilmove E. faecalis, TotalFill BC Sealer (Brasseler SAD) i AH Plus (Dentsply) su pokazali bolju kratkoročnu antibakterijsku učinkovitost od BioRoot RCS-a (Septodont) i MTA Fillapexa (Angelus). |
| Abstract (english) | Aim: The aims of the studies were to compare the antimicrobial efficacy of bioceramic root canal sealers and to examine their antibacterial efficacy on planktonic cells and young biofilms of E. faecalis.
Materials and methods: In the first part of the study, three electronic databases were searched: MEDLINE via OvidSP platform, Embase via OvidSP platform and Web of Science. Studies were included irrespective of research design, type of publication, and language. Reporting quality was assessed by two authors independently. In the second part of the study, the bacterial suspension was placed on the surface of freshly mixed sealers and one or three days set sealer in vertically held microtiter plates. After incubation of 2, 5, 20 and 60 minutes, Tripticase Soy Broth was added to each well of the microtiter plate. After serial dilution, colony forming units were counted. In the third part of the study, bacterial biofilms were grown on filter paper discs placed on agar plates and, after incubation, the discs were covered with four root canal sealers. After 60 minutes, the sealers were removed and the discs were transferred to tubes containing phosphate buffered saline. After serial dilution, colony forming units were counted.
Results: The first part of the study included 37 studies that examined the antimicrobial efficacy of bioceramic sealers. It is not possible to make a comparison of the results from studies and to give a clear conclusion about their comparative activity because the studies used heterogeneous source and age groups of microorganisms, setting and contact times of sealers and antimicrobial tests.
In the second part of the study bioceramic materials showed significantly better efficacy than the control group and resin-based material (p <0.05). TotalFill BC Sealer (Brasseler USA) showed the highest efficacy, comparable to BioRoot RCS (Septodont) (p> 0.05), followed with MTA Fillapex (Angelus) (p <0.05). Freshly prepared materials had similar efficacy as sealers set for three days (p> 0.05), but better than sealers set for one day (p <0.05). All sealers exibithed the highest efficacy after 20 minutes of contact, independly sealers were freshly mixed or set for one or three days.
In the third part of the study, there was no significant difference between TotalFill BC Sealer (Brasseler USA) and AH Plus (Dentsply) (p = 0.386). Both sealers showed better antibacterial efficacy than BioRoot RCS (Septodont) and MTA Fillapex (Angelus) (p <0.001).
Conclusion: Since the studies included in the systematic review are highly heterogeneous, it is not possible to give a conclusion about which sealer has the best performance or recommendations for clinical practice because the studies were conducted in different ways, which makes meta-analysis futile. It is necessary to uniform the methodological approach, clearly define the materials classification and conduct studies on humans in order to be able to make recommendations for clinical practice. In a study of efficacy of bioceramic materials on E. faecalis planktonic cells, bioceramic sealers showed better efficacy compared to commercially used resin-based sealer. All sealers exhibited strongest efficacy at 20 minutes of contact time, independly sealer was freshly mixed or one or three day set. In a study of the efficacy of bioceramic sealers on young E. faecalis biofilms, TotalFill BC Sealer (Brasseler USA) and AH Plus (Dentsply) showed better short-term antibacterial efficacy than BioRoot RCS (Septodont) and MTA Fillapex (Angelus). |
