prikaz prve stranice dokumenta Effect of aerobic interval training on pathological remodelling and mitochondrial dysfunction in the post-infarction failing rat heart
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doctoral thesis
Effect of aerobic interval training on pathological remodelling and mitochondrial dysfunction in the post-infarction failing rat heart
2015. urn:nbn:hr:171:643734
Kraljević, Jasenka
University of Split
School of Medicine

Cite this document

Kraljević, J. (2015). Effect of aerobic interval training on pathological remodelling and mitochondrial dysfunction in the post-infarction failing rat heart (Doctoral thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:643734

Kraljević, Jasenka. "Effect of aerobic interval training on pathological remodelling and mitochondrial dysfunction in the post-infarction failing rat heart." Doctoral thesis, University of Split, School of Medicine, 2015. https://urn.nsk.hr/urn:nbn:hr:171:643734

Kraljević, Jasenka. "Effect of aerobic interval training on pathological remodelling and mitochondrial dysfunction in the post-infarction failing rat heart." Doctoral thesis, University of Split, School of Medicine, 2015. https://urn.nsk.hr/urn:nbn:hr:171:643734

Kraljević, J. (2015). 'Effect of aerobic interval training on pathological remodelling and mitochondrial dysfunction in the post-infarction failing rat heart', Doctoral thesis, University of Split, School of Medicine, accessed 01 November 2024, https://urn.nsk.hr/urn:nbn:hr:171:643734

Kraljević J. Effect of aerobic interval training on pathological remodelling and mitochondrial dysfunction in the post-infarction failing rat heart [Doctoral thesis]. Split: University of Split, School of Medicine; 2015 [cited 2024 November 01] Available at: https://urn.nsk.hr/urn:nbn:hr:171:643734

J. Kraljević, "Effect of aerobic interval training on pathological remodelling and mitochondrial dysfunction in the post-infarction failing rat heart", Doctoral thesis, University of Split, School of Medicine, Split, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:171:643734

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Metadata
TitleEffect of aerobic interval training on pathological remodelling and mitochondrial dysfunction in the post-infarction failing rat heart
Title (croatian)Učinak aerobnog intervalnog treninga na patološko remodeliranje i mitohondrijsku disfunkciju u štakora s poslijeinfarktnim zatajenjem srca
AuthorJasenka Kraljević
MentorJasna Marinović Ljubković (mentor)
Committee memberJanoš Terzić (predsjednik povjerenstva)
Committee memberStjepan Gamulin (član povjerenstva)
Committee memberDamir Fabijanić (član povjerenstva)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2015, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Internal Medicine
Universal decimal classification
(UDC)		616 - Pathology. Clinical medicine
Abstract
Aims: Following a large myocardial infarction (MI), remaining viable muscle often undergoes pathological remodelling and progresses towards chronic heart failure (CHF). Mitochondria may also be affected by this process and, due to their functional importance, likely contribute to the progression of the disease. Aerobic interval training (AIT) has been shown effective in diminishing pathological myocardial transformation, but the effects of AIT on mitochondrial function in hearts undergoing remodelling are not known. Methods and Results: Adult female Sprague-Dawley rats were randomized to either 8 weeks of aerobic interval treadmill running (5 days/week), which started 4 weeks after left coronary artery ligation (MI-Trained), or a sedentary group (MI-Sedentary). Echocardiography was performed before and after the 8-week period, at which point the left ventricles (LV) were also harvested. Twelve weeks after surgery, MI-Sedentary rats had significantly lower LV fractional shortening compared to MI-Trained. Complex I – dependent respiration assessed in isolated LV mitochondria was decreased by ~37% in MI-Sedentary and 17% in MI-Trained (group differences p<0.05), compared to sham-operated animals. This was paralleled with diminished ATP-production and increased degree of protein oxidation in MI-Sedentary rats. The enzymatic activity of complex I was also decreased to the greater extent in MI-Sedentary than in MI-Trained animals, with no evidence of its reduced expression. When complex II substrate was used, no differences among the three groups were observed. Conclusions: Exercise reduces the left ventricle contractile deterioration in post-infarction heart failure and alleviates the extent of mitochondrial dysfunction, which is paralleled with preserved complex I activity.
Abstract (croatian)
Uvod: Nakon opsežnog infarkta miokarda (IM) u dijelu srčanog mišića izvan zahvaćenog područja dolazi do patološkog remodeliranja koje ako napreduje dovodi do razvoja kroničnog srčanog zatajenja. Patološko remodeliranje obuhvaća makroskopske i mikroskopske promjene na razini srčanog mišića. Remodeliranje na razini mitohondrija vjerojatno je važni čimbenik napredovanja bolesti zbog njihove ključne uloge u energijskom metabolizmu stanice. Aerobna intervalna tjelovježba učinkovito ublažava patološku transformaciju miokarda iako još uvijek nisu dovoljno istraženi mehanizmi preko kojih se to događa. Materijali i metode: Ženke štakora Sprague-Dawley randomizirane su u dvije skupine: jedna je skupina 4 tjedna nakon podvezivanja lijeve koronarne arterije bila podvrgnuta aerobnoj intervalnoj tjelovježbi koja se sastojala od trčanja na pokretnoj traci (5 dana/tjedan) dok je druga tijekom istog razdoblja živjela sedentarnim životom. Prije i poslije perioda tjelovježbe od 8 tjedana učinjeno je ehokardiografsko testiranje. Nakon provedenih ispitivanja životinje su bile žrtvovane, a iz srca izolirani dijelovi lijevog ventrikula izvan područja zahvaćenog infarktom. Rezultati: 12 tjedana nakon kirurškog zahvata sedentarne su životinje imale značajno nižu frakciju skraćenja lijevog ventrikula u usporedbi s treniranim životinjama. U mitohondrijima izoliranim iz lijevog ventrikula respiracija preko kompleksa I bila je 37% manja u sedentarnih i 17% manja u treniranih životinja (statistička razlika p<0.05) u usporedbi s kontrolnom skupinom (Sham štakori). Uz ove promjene u sedentarnih životinja postoji i smanjena proizvodnja ATP-a i povećana oksidacija proteina. Enzimska aktivnost kompleksa I respiracijskog lanca elektrona značajno je smanjena u sedentarnih u usporedbi s treniranim životinjama bez razlike u ekspresiji proteina kompleksa I. Nema statistički značajne razlike među grupama kada se respiracija odvijala uz supstrate za respiraciju preko kompleksa II. Zaključci: Tjelovježba ublažava propadanje kontraktilne funkcije lijevog ventrikula i ublažava mitohondrijsku disfunkciju uz očuvanu aktivnost kompleksa I u srčanom mišiću nakon infarkta miokarda
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:171:643734
Study programmeTitle: Evidence-Based Clinical Medicine
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-05-15 11:44:33