| Abstract | Objectives: The aim was to assess the immunohistochemical expression of PDPN/D2-40 in the
three most common types of renal cell carcinoma, clear cell renal cell carcinoma (CCRCC),
papillary renal cell carcinoma (PRCC) and chromophobe renal cell carcinoma (ChRCC).
Additionally, we wanted to determine if there is a correlation between cancer aggressivity and
metastatic potential and an increase of PDPN expression in aforementioned tumors.
Materials and methods: Our study included 15 RCC samples from patients operated at
Urology Department of University Hospital Split and diagnosed at the Clinical Institute for
Pathology, Forensic Medicine and Cytology at the same hospital, in the period from 1st of
January 2018 till 31st of December 2018. The patient9s data, including patient9s gender and age
at the time of diagnosis were collected and pathology report operation procedure provided
information on histological tumor type, size of the tumor, as well as presence of necrosis and
lymphovascular invasion. PDPN expression was determined via immunohistochemical
analysis.
Results: 80% of patients were male. The average age of male patients at the time of diagnosis
was 69 years and 37 years for female patients, which was statistically significant (P<0.001).
There was no statistically significant difference between studied groups in regard to tumor size
(P=0.530), presence of tumor necrosis (P=0.489) or lymphovascular invasion (P=0.438). The
non-clear cell renal cell carcinoma group had slightly higher immunohistochemical expression
of PDPN which was not statistically significant (P=0.554). The clear cell RCC group had higher
number of PDPN positive lymphatics (23 ± 9) compared to non-clear cell RCC group (14 ± 9),
which wasn9t statistically significant (P=0.141). There is a negative correlation between
immunohistochemical expression of PDPN in tumor cells and the presence of tumor necrosis
and tumor size, but without statistical significance (r=-0.151; P=0.589) (r=-0.202; P=0.468).
There was statistically significant negative correlation between number of positive lymphatics
in the tumor surrounding tissue and tumor size (r=-0.588; P=0.021)
Conclusion: No statistically significant correlation between PDPN expression and the
histological characteristics of the tumor, PDPN invasiveness, tumor necrosis, or size were
found. Additional studies are needed to clarify the role of PDPN in relation to renal cell
carcinoma characteristics. |
| Abstract (croatian) | Ciljevi: odrediti imunohistokemijski izražaj PDPN/D2-40 u tri najčešća histološka tipa karcinoma bubrežnih stanica: svijetlo stanični karcinom (CCRCC), papilarni karcinom (PRCC) i kromofobni karcinom (ChRCC), te odrediti postoji li korelacija između metastatskog potencijala ispitivanih histoloških tipova tumora i PDPN imunohistokemijskog izražaja. Materijali i metode: u studiju je uključeno 15 uzoraka karcinoma bubrega, operiranih na Klinici za urologiju Kliničkog bolničkog centra Split, a čija patohistološka dijagnoza je postavljena na Kliničkom zavodu za patologiju, sudsku medicinu i citologiju iste bolnice, u razdoblju od 01.01.2018. godine do 31.12.2018. godine. Podaci o pacijentima, uključujući spol, dob u vrijeme postavljanja dijagnoze, tip operacijskog zahvata, histološki tip i veličina tumora, te prisutnost nekroze i limfovaskularne invazije, zabilježeni su iz patoshistološkog nalaza. Imunohistokemijski izražaj PDPN/D2-40 određen je uporabom HSCORE metode. Rezultati: 80% pacijenata je bilo muškoga spola. Prosječna dob muških pacenata u vrijeme postavljanja dijagnoze je bila 69 godina, a za žene 37 godina što je bilo statistički značajno (P< 0,001). Nije bilo statistički značajne razlike između ispitivanih skupina obzirom na veličinu tumora (P=0,530), prisutnost nekroze (P=0,489) ili limfovaskularne invazije (P=0,438). U skupini ne-svijetlostaničnih RCC imunohistokemijski izražaj PDPN/D2-40 je bio veći u usporedbi sa CCRCC skupinom, ali bez statističke značajnosti (P=0,554). U skupini CCRCC bilo je više PDPN/D2-40 pozitivnih limfnih žila (23 ± 9) u usporedbi s ne-svijetlostaničnom RCC skupinom (14 ± 9), bez statističke značajnosti (P=0,141). Postoji negativna korelacija između imunohistokemijskog izražaja PDPN/D2-40 u tumorskim stanicama i tumorske nekroze i veličine tumora, bez statističke značajnosti (r=-0,151; P=0,589) (r=-0,202; P=0,468). Postoji statistički značajna negativna korelacija između broja PDPN/D2-40 pozitivnih limfnih žila u tkivu koje okružuje tumor i veličine tumora (r=-0,588; P=0,021). Zaključak: Nije nađena statistički značajna povezanost imunohistokemijskog izražaja PDPN/D2-40 i histoloških karakteristika tumora, limfovaskularne invazije, tumorske nekroze ni veličine tumora. Dodatna istraživanja su potrebna kako bi se rasvijetlila uloga PDPN u razvoju i metastatskom potencijalu RCC-a. |
