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master's thesis
Detection of COVID-19 infection in nasal breath by gas chromatography coupled ion mobility spectrometry (GC-IMS)
2024. urn:nbn:hr:171:342670
Will, Margarete Mirjam
University of Split
School of Medicine

Cite this document

Will, M. M. (2024). Detection of COVID-19 infection in nasal breath by gas chromatography coupled ion mobility spectrometry (GC-IMS) (Master's thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:342670

Will, Margarete Mirjam. "Detection of COVID-19 infection in nasal breath by gas chromatography coupled ion mobility spectrometry (GC-IMS)." Master's thesis, University of Split, School of Medicine, 2024. https://urn.nsk.hr/urn:nbn:hr:171:342670

Will, Margarete Mirjam. "Detection of COVID-19 infection in nasal breath by gas chromatography coupled ion mobility spectrometry (GC-IMS)." Master's thesis, University of Split, School of Medicine, 2024. https://urn.nsk.hr/urn:nbn:hr:171:342670

Will, M. M. (2024). 'Detection of COVID-19 infection in nasal breath by gas chromatography coupled ion mobility spectrometry (GC-IMS)', Master's thesis, University of Split, School of Medicine, accessed 20 November 2024, https://urn.nsk.hr/urn:nbn:hr:171:342670

Will MM. Detection of COVID-19 infection in nasal breath by gas chromatography coupled ion mobility spectrometry (GC-IMS) [Master's thesis]. Split: University of Split, School of Medicine; 2024 [cited 2024 November 20] Available at: https://urn.nsk.hr/urn:nbn:hr:171:342670

M. M. Will, "Detection of COVID-19 infection in nasal breath by gas chromatography coupled ion mobility spectrometry (GC-IMS)", Master's thesis, University of Split, School of Medicine, Split, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:171:342670

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Metadata
TitleDetection of COVID-19 infection in nasal breath by gas chromatography coupled ion mobility spectrometry (GC-IMS)
Title (croatian)Otkrivanje infekcije COVID-19 u nosnom dahu pomoću plinske kromatografije spojene s ionskom spektrometrijom (GC-IMS)
AuthorMargarete Mirjam Will
MentorJohannes Brachmann (mentor)
Committee memberGeorg Gerhard Grabenbauer (predsjednik povjerenstva)
Committee memberMarino Vilović (član povjerenstva)
Committee memberIvana Pavlinac Dodig (član povjerenstva)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2024-09-04, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Internal Medicine
Abstract
Objectives: This study was aimed to assess whether GC-IMS can be used for the detection of SARS-CoV-2. Methods: We performed a non-randomized controlled study measuring VOC clusters in the nasal breath of 91 hospitalized patients with a GC-IMS device. The study was conducted at the Regiomed hospital Coburg between April and July 2022. Patients were divided into two groups, according to their PCR test result (CT <40) and were measured with GC-IMS not longer than 24 hours after their PCR test. Participants needed to give their written informed consent and meet the inclusion criteria for our study. Statistical analysis in form of data descriptive tests (Mann-Whitney-U, Pearson’s Chi-squared and Fisher’s exact) and discriminant cluster analysis of the measured VOCs (stepwise canonical approach, leave-one-out cross validation) was performed using IBM SPSS 25. Results: There were no statistically significant findings regarding age distribution among genders and study groups. 51 clusters were measured by GC-IMS in the nasal breath of patients; 2 clusters showed statistically relevant differences among the study groups. GC-IMS correctly classified 68.1% of patients regarding their PCR test result and 65.9% (64.8% after cross validation) regarding their contagiousness (CT <30). Based on that we calculated a sensitivity of 66.1% and specificity of 71.9% for GC-IMS. Conclusion: GC-IMS can detect SARS-CoV-2 by measuring the VOC composition of patients’ nasal breath with a sensitivity similar to those of antigen tests. It may therefore serve as a cost-effective, fast and non-invasive screening method, especially at places where mass testing is required.
Abstract (croatian)
Ciljevi: Ova studija je imala za cilj procijeniti može li se GC-IMS koristiti za otkrivanje SARS-CoV-2. Materijali i metode: Proveli smo nekontroliranu, nenamjensku studiju mjereći VOC skupine u nosnom dahu 91 hospitaliziranog pacijenta pomoću GC-IMS uređaja. Studija je provedena u bolnici Regiomed u Coburgu između travnja i srpnja 2022. Pacijenti su bili podijeljeni u dvije skupine, prema rezultatu njihovog PCR testa (CT <40) i mjerili su se s GC-IMS ne duže od 24 sata nakon PCR testa. Sudionici su trebali dati svoj pisani informirani pristanak i ispuniti kriterije inkluzije za našu studiju. Statistička analiza u obliku deskriptivnih testova podataka (Mann-Whitney U, Pearsonov hi-kvadrat i Fisherova egzaktna) i diskriminantna klasterska analiza izmjerenih VOC-ova (korak po korak kanonski pristup, leave-one-out unakrsna validacija) provedena je korištenjem IBM SPSS 25. Rezultati: Nisu zabilježeni statistički značajni nalazi u vezi s distribucijom dobi među spolovima i istraživačkim skupinama. 51 klaster mjerena je metodom GC-IMS u nosnom dahu pacijenata; 2 klastera pokazala su statistički relevantne razlike među istraživačkim skupinama. GC-IMS ispravno je klasificirao 68,1% pacijenata prema rezultatu njihovog PCR testa i 65,9% (64,8% nakon unakrsne validacije) prema njihovoj zaraznosti (CT <30). Na temelju toga izračunali smo senzitivnost od 66,1% i specifičnost od 71,9% za GC-IMS. Zaključci: GC-IMS može otkriti SARS-CoV-2 mjereći sastav VOC-a u nosnom dahu pacijenata sa senzitivnošću sličnom onima antigenskih testova. Stoga može poslužiti kao isplativa, brza i neinvazivna metoda skrininga, posebno na mjestima gdje je potrebno masovno testiranje.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:171:342670
Study programmeTitle: Medical Studies in English
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-09-04
Terms of useLicence In copyright icon
Created on2024-09-04 07:09:54