Aim of the study: In non-small cell lung cancer (NSCLC) to determine the expression of GLUT1 in the primary tumor and metastatic lymph node, the expression of HIF-1α and HIF1A mRNA in the primary tumor and compare them with the preoperative SUVmax value in PET/CT using FDA, histological type and other clinical and pathological parameters. Material and methods: The sample includes 48 patients with NSCLC who underwent lobectomy/pulmectomy with mediastinal lymphadenectomy at University Hospital Split from 2013 to 2018. Data on FDG accumulation and clinicopathological parameters were collected from medical records. The histologic slides were cut from paraffin blocks of tumor tissue and metastatic lymph node. They were stained immunohistochemically for GLUT1 and microscopically analyzed using the HSCORE method. They were manually stained for HIF-1α using the immunofluorescence method, and the percentage of tumor cells that showed nuclear or nuclear/cytoplasmic staining for HIF-1α was determined. The difference in HIF-1A mRNA of adenocarcinoma and squamous cell carcinoma was analyzed using the qPCR method. The results were statistically analyzed and a significant difference was determined at the p = 0.05. Results: 32 patients were men and 16 women, 34 (70.83%) with adenocarcinoma and 14 (29.16%) with squamous cell carcinoma. In squamous cell carcinoma, GLUT1 expression in the primary tumor and metastatic lymph node was higher than in adenocarcinoma (p<0.0001 and p<0.0196, respectively). In squamous cell carcinoma, there was a higher percentage of cells with nuclear and nuclear/cytoplasmic expression of HIF-1α than in adenocarcinoma (p = 0.0025 and p = 0.0038, respectively). In squamous cell carcinoma, an association between nuclear and nuclear/cytoplasmic expression of HIF-1α was established (p=0.0004). No statistically significant difference was found in the expression of HIF-1α depending on the stage of the disease. The nuclear/cytoplasmic expression of HIF-1α was higher in men than in women (p=0.0089). GLUT1 expression was associated with nuclear and nuclear/cytoplasmic expression of HIF-1α (p=0.0392 and p=0.0498, respectively). No significant difference was found in HIF-1A mRNA in relation to the histological type or SUVmax of the primary tumor. Conclusion: Proven differences in the expression of GLUT1, HIF-1α and SUVmax in relation to the type of NSCLC confirm the metabolic heterogeneity between adenocarcinoma and squamous cell lung cancer.