INTRODUCTION: In classical Hodgkin lymphoma (cHL), Hodgkin/Reed-Sternberg (HRS) cells are surrounded by an abundant, ineffective tumor immune microenvironment (TIM). In the TIM there are regulatory T lymphocytes (Treg) responsible for tolerance, of which tTreg originating from the thymus express FOXP3. HRS often contain the Epstein-Barr virus (EBV) monoclonal genome, whose latent proteins affect the composition of TIM. We analyzed the number and type of T lymphocytes expressing the regulatory marker FOXP3 with respect to EBV status, subtype, and outcome measures. MATERIALS AND METHODS: The sample includes 182 patients with cHL diagnosed in the period 1997-2018. and was treated at University Hospital Split. Slides were cut from paraffin blocks of tumor tissue and stained by in situ hybridization for EBER for evidence of EBV and double immunohistochemical staining for FOXP3 and CD4 or CD8 for microscopic analysis of T cell phenotypes in 1 mm2 . The statistically significant difference was p<0.05. RESULTS: Most patients, 124 (68.1%) had nodular sclerosis (NS) or mixed cellularity (MC), 4422 (24.2%). EBV-positive cHL was confirmed in 28% of cases and was associated with older age (p< 0.001), MC (p< 0.001) and death (p=0.038). There is a difference in the number of FOXP3 cells (p=0.035), CD8 cells (p=0.001) and FOXP3/CD4 cells (p=0.041) in NS and MC with regard to EBV status. Patients younger than 26.5 years had more CD4 T lymphocytes in TIM than patients aged 26.5-36 years (p=0.009). Higher-than-median values of FOXP3 and CD4 lymphocytes were associated with longer progression-free time (PFS) (p=0.001 and p=0.046, respectively). CONCLUSION: EBV-positive status of cHL affects the composition of T cell phenotypes in TIM, among which CD4 and FOXP3 counts are prognostically valuable.