Aim: The goal of the research is to determine the epidemiological characteristics of botulism in the Republic of Croatia in the period from 1995. to 2015. Methods: The retrospective study was taken and the data on the number of patients and deaths from tetanus in the period of 1995-2015 in Croatia were analyzed. The data considering age, gender and geographical location of disease occurrence were also analyzed. Results: The study confirmed the reduction of morbidity of botulism in the Republic of Croatia in the period, but also a higher incidence of botulism than in most neighboring countries for which data are available in the literature. In the last twenty years the average morbidity rate was 1,81/1 000 000 population. A higher incidence of botulism was observed in male patients (58%), and affected people belonged to all age groups. The highest number of cases was reported in Splitsko-dalmatinska county (24) and Zagreb town (13), and the only one death was recorded in the Krapinsko-zagorska county (2002). Conclusion: Recorded downward trend in the incidence of botulism in Croatia indicates a high probability that the measures of education programs about preventing this type of food poisoning were successful.