OBJECTIVE: To analyze demographic imprint of children treated in Pediatric intensive care unit (PICU) of Split University hospital, epidemiology of infections and its connection to the usage of medical equipment. Furthermore, objective was to determine incidence of certain microbiological isolates, test its sensitivity on antibiotics and answer the question if these indicators vary depending on demographic imprints of children. PATIENTS AND METHODS: For the intent of obtaining the data, medical documentation created in the PICU of Split University Hospital was analyzed retrospectively. Equally, for the purpose of research, microbiological findings were adopted from Microbiological department of Split Univerity Hospital. RESULTS AND CONCLUSION: Newborns had the highest risk for developing infections and had the most cases of bacteremia. Within blood culture, urine culture and tracheal wash gram negative bacteria were the most common isolates, while the fungus were isolated in lower rate than expected. Once we analyzed microbiological sensitivity of the most resistant isolates within children treated in PICU of Split University Hospital, we have fonud the worrisome high resistence of P. aeruginosa for carbapenems. The research shown that the usage of various invasive procedures which are applied at PICU were associated with infection development.