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master's thesis
Promjene debljine epidermisa i kožne inervacije uzrokovane dijabetesom tijekom starenja
2016. urn:nbn:hr:171:504009
Viđak, Marin
University of Split
School of Medicine
Department of Anatomy, Histology and Embryology

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Viđak, M. (2016). Promjene debljine epidermisa i kožne inervacije uzrokovane dijabetesom tijekom starenja (Master's thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:504009

Viđak, Marin. "Promjene debljine epidermisa i kožne inervacije uzrokovane dijabetesom tijekom starenja." Master's thesis, University of Split, School of Medicine, 2016. https://urn.nsk.hr/urn:nbn:hr:171:504009

Viđak, Marin. "Promjene debljine epidermisa i kožne inervacije uzrokovane dijabetesom tijekom starenja." Master's thesis, University of Split, School of Medicine, 2016. https://urn.nsk.hr/urn:nbn:hr:171:504009

Viđak, M. (2016). 'Promjene debljine epidermisa i kožne inervacije uzrokovane dijabetesom tijekom starenja', Master's thesis, University of Split, School of Medicine, accessed 29 November 2024, https://urn.nsk.hr/urn:nbn:hr:171:504009

Viđak M. Promjene debljine epidermisa i kožne inervacije uzrokovane dijabetesom tijekom starenja [Master's thesis]. Split: University of Split, School of Medicine; 2016 [cited 2024 November 29] Available at: https://urn.nsk.hr/urn:nbn:hr:171:504009

M. Viđak, "Promjene debljine epidermisa i kožne inervacije uzrokovane dijabetesom tijekom starenja", Master's thesis, University of Split, School of Medicine, Split, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:171:504009

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Metadata
TitlePromjene debljine epidermisa i kožne inervacije uzrokovane dijabetesom tijekom starenja
Title (english)Changes in epidermal thickness and cutaneous innervation during maturation in long-term diabetes
AuthorMarin Viđak
MentorLivia Puljak (mentor)
GranterUniversity of Split
School of Medicine
(Department of Anatomy, Histology and Embryology)
Split
Defense date and country2016, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Cytology, Histology and Embryology
Abstract
Cilj istraživanja: Smanjenje broja perifernih živčanih vlakana u pacijenata s kroničnim dijabetesom mellitusom (DM) povezano je sa simptomima neuropatije, razvojem boli, ulceracija stopala i amputacijama donjih ekstremiteta. Cilj ovog rada je bio procijeniti kožne promjena, uključujući promjene u debljini epidermisa na šapi i u gustoći intraepidermalnih živčanih vlakana (engl. intraepidermal nerve fibers – IENF) u dugotrajnom dijabetesu u štakora, 6 i 12 mjeseci nakon izazivanja dijabetesa. Materijali i metode: Debljina epidermisa i gustoća IENF istraživana je u Sprague-Dawley dijabetičkih štakora 6 i 12 mjeseci nakon izazivanja dijabetesa streptozotocinom. Debljina epidermisa je procijenjena hematoksilinskim i eozinskim bojanjem. Periferna živčana vlakna bojana su poliklonalnim antiserumom za PGP 9,5. Uspješnost izazivanja dijabetesa provjeravana je mjerenjem glukoze u plazmi i tjelesne mase štakora tijekom eksperimenta. Rezultati: Ovo je istraživanje pokazalo kako je dugotrajni dijabetes izazvan streptozotocinom u Sprague-Dawley štakora karakteriziran značajnim stanjenjem epidermisa i smanjenjem broja intraepidermalnih živčanih vlakana 6 i 12 mjeseci nakon indukcije dijabetesa. Zaključak: Štakori s dugotrajnim dijabetesom tipa 1 izazvanim streptozotocinom imaju morfometrijske promjene kože koje su karakteristične za dijabetičku neuropatiju i zbog toga se mogu koristiti za buduća farmakološka istraživanja.
Abstract (english)
Aim: Peripheral nerve fiber depletion in patients with chronic diabetes mellitus (DM) was linked to neuropathic symptoms, development of pain, foot ulcerations and lower extremity amputation. The aim of this study was to analyze cutaneous changes, including paw epidermal thickness and intraepidermal nerve fiber (IENF) density in long-term diabetes, in rats 6 months and 12 months after induction of diabetes. Materials and methods: Epidermal thickness and IENF density were studied in Sprague-Dawley diabetic rats 6 months and 12 months after diabetes induction with streptozotocin. Epidermal thickness was evaluated using hematoxylin and eosin staining. Peripheral nerve fibers were stained with polyclonal antiserum against protein gene product 9.5 (PGP 9.5). Successful diabetes induction was validated by measuring plasma glucose and body mass regularly throughout the experiment. Results: This study showed that long-term diabetes, induced in Sprague-Dawley rats with streptozotocin, is characterized with significant epidermal thinning and reduction of intraepidermal nerve fibers, 6 months and 12 months after induction of diabetes. Conclusion: Long-term studies of streptozotocin models of diabetes could be used for making normative IENF densities that can be later used as age-dependent normative values for studying new treatment modalities.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:504009
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2016-10-27 11:49:05