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master's thesis
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CYTOTOXIC AND GENOTOXIC EFFECTS OF DIFFERENT KINDS OF TOOTHPASTE WITH AND WITHOUT FLUORIDE ON ORAL MUCOSA CELLS
Tanja Gović (2017)
University of Split
School of Medicine
Endodoncy and restorative dentistry
Metadata
TitleCITOTOKSIČNI I GENOTOKSIČNI UČINAK ZUBNIH PASTA S FLUOROM I BEZ FLUORA NA STANICAMA BUKALNE SLUZNICE
AuthorTanja Gović
Mentor(s)Antonija Tadin (thesis advisor)
Abstract
Cilj istraživanja: Paste za zube sadrže mnoge potencijalno štetne sastojke, uključujući neke koje mogu dovesti do ozbiljnih dugoročnih zdravstvenih problema. Svakodnevna upotreba proizvoda za oralnu zdravstvenu zaštitu je velika što naglašava potrebu da zdravstveni djelatnici i potrošači budu informirani o potencijalnim prednostima i rizicima povezanim s tim proizvodima. Ovo istraživanje bilo je usmjereno na procjenu mogućih DNK oštećenja u oralnim epitelnim stanicama kod sudionika koji su bili izloženi zubnim pastama s fluorom u usporedbi s onima bez fluora. Materijali i metode: Četrdeset volontera izabrano je među studentima dentalne medicine i podijeljeno u dvije eksperimentalne skupine. Svaka grupa koristila je prvo zubnu pastu bez fluora kroz prva dva mjeseca, nakon čega je slijedila uporaba fluoridiranih vrsta istog proizvođača kroz isto vrijeme korištenja. Oralne epitelne stanice su uzorkovane na početku te 30, 60, 90 i 120 dana od početka uporabe ispitanih vrsta zubnih pasta. Kromosomska oštećenja analizirana su mikronukleus testom. Rezultati: Obje testirane zubne paste s fluorom pokazale su povećane parametre genotoksičnosti i citotoksičnosti nakon trideset dana korištenja. Većina ispitivanih parametara se smanjila se nakon 60 dana i nisu se značajno razlikovali od rezultata dobivenih u vrijeme kada su se koristilezubne paste bez fluorida. Zaključak: Na temelju rezultata može se zaključiti kako uporaba pasta za zube s fluorom može izazvati prolaznu i ograničenu toksičnost, međutim dugoročno biološki beznačajnu.
KeywordsMouth Mucosa Toothpastes Fluorides Micronucleus Tests DNA Damage
Parallel title (English)CYTOTOXIC AND GENOTOXIC EFFECTS OF DIFFERENT KINDS OF TOOTHPASTE WITH AND WITHOUT FLUORIDE ON ORAL MUCOSA CELLS
GranterUniversity of Split
School of Medicine
Lower level organizational unitsEndodoncy and restorative dentistry
PlaceSplit
StateCroatia
Scientific field, discipline, subdisciplineBIOMEDICINE AND HEALTHCARE
Dental Medicine
Endodonics and Restorative Dentistry
Study programme typeuniversity
Study levelintegrated undergraduate and graduate
Study programmeDental Medicine
Academic title abbreviationdr. med. dent.
Genremaster's thesis
Language Croatian
Defense date2017
Parallel abstract (English)
Aim: Toothpastes contain many potentially harmful ingredients, including some that can lead to serious long-term health problems. Everyday use of oral health care products has increased, highlighting a need for healthcare clinicians and consumers alike to be informed of the potential benefits and risks associated with these products. This study aimed to evaluate possible DNA damage to oral epithelial cells in participants exposed to fluoride kinds of toothpaste considering the effect of non-fluoride kinds of toothpaste. Materials and methods: Forty volunteers were found among students of dental medicine and assigned into two experimental groups. Each group used regular toothpaste without fluoride for initial two months, followed by the use of fluoride kinds of toothpaste of the same brand for the next two months. The oral epithelial cells were sampled at baseline and 30, 60, 90 and 120 days after the beginning of the use of tested types of toothpaste. Chromosomal damage was analysed by micronucleus assay. Results: Both tested fluoride kinds of toothpaste showed increased parameters of genotoxicity and cytotoxicity after thirty days of use. Most of the studied parameters decreased after 60 days of use and were not significantly different from the results obtained at the time when the participants used toothpaste without fluoride. Conclusion: Based on the results, we can conclude that the use of toothpaste with fluoride may cause transient limited toxicity, long-term biologically insignificant.
Parallel keywords (Croatian)Sluznica usta Zubne paste Floridi Testovi mikronukleusa Oštećenja DNK
Resource typetext
Access conditionOpen access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:171:758552