Objective: The aim of this study was to determine whether there is a statistically significant difference between measurements with Goldmann aplannation tonometry with and without fluorescein. Materials and methods: The research was conducted at the Clinic for Ophthalmology of University Hospital of Split. There were 39 patients involved and 78 eyes were examined. The time spent collecting the data was one calendar month. Each subject was put anesthetic (tetracaine) in the eye and then we started with the eye pressure measurements. For each subject we measured pressure three times without fluorescein using red-free light. Then we measured pressure for each subject three times using fluorescein (disposable band) and cobalt blue light. As a statistically significant difference the significance level p < 0.05 was used. Results: Of the 39 respondents involved in this study, 19 (48.72%) were women and 20 (51.28%) males. The mean age of the respondents was 71.74 ± 8.27. By comparing three consecutive intraocular pressure measurements (IOP), we found that the mean value of each subsequent measurement, whether for a series of measurements without or with fluorescein, was statistically significantly lower. To compare the measured pressure between the series measured without flurescein and with the fluorescein we did not take the mean value, but we compared each non-fluorescein and fluorescein sequential measurement. There was no statistically significant difference between individual sequential measurements without and with fluorescein. Then we proved that the IOT CCD measurements (the pressure measurement with the axis setting prism in the direction of the astigmatism axis) statistically differ significantly from the first measurements with and without fluorescein. There was a negative correlation between corneal astigmatism and the difference between corrected IOT CCD measurement and the first IOT measurement without fluorescein. The higher the corneal astigmatism, the measurement without the correction of the axis of the prism was more and more overestimated by the IOT compared to the IOT CCD measurement. There was also a positive correlation between the astigmatism axis deviation from the horizontal axis and the difference between corrected IOT (IOT CCD) measurement and the first measurement without fluorescein. This leads to the conclusion that the greater the deviation of the axis of astigmatism from the horizontal axis, the first measurement without the correction of the axis of the prism increasingly underestimated the IOT compared to the IOT CCD measurement. Conclusion: There is no statistically significant difference between the measurement of Goldmann's aplastic tonometers with and without fluorescein. Consecutive IOP measurements in a few seconds intervals will decrease its value. There is a positive correlation between the deviation of the astigmatism axis and the difference between the corrected IOP measurement and the first measurement without fluorescein. There is a negative correlation between the astigmatism axis deviation and the difference between the corrected IOP and the first measurement without fluorescence. In view of that, no fluorescein should be used for further clinical procedures, it is recommended to use Goldmann applanation tonometry without fluorescein using red-free light.Tonometry, Ocular Intraocular Pressure Fluorescein