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QUANTITATIVE DETERMINATION OF SYNTHETIC CATHINONES
Martina Bošnjak (2018)
University of Split
School of Medicine
Cite this document...

Bošnjak, M. (2018). KVANTITATIVNO ODREĐIVANJE SINTETSKIH KATINONA (Master's thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:674539

Bošnjak, Martina. "KVANTITATIVNO ODREĐIVANJE SINTETSKIH KATINONA." Master's thesis, University of Split, School of Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:171:674539

Bošnjak, Martina. "KVANTITATIVNO ODREĐIVANJE SINTETSKIH KATINONA." Master's thesis, University of Split, School of Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:171:674539

Bošnjak, M. (2018). 'KVANTITATIVNO ODREĐIVANJE SINTETSKIH KATINONA', Master's thesis, University of Split, School of Medicine, accessed 25 March 2019, https://urn.nsk.hr/urn:nbn:hr:171:674539

Bošnjak M. KVANTITATIVNO ODREĐIVANJE SINTETSKIH KATINONA [Master's thesis]. Split: University of Split, School of Medicine; 2018 [cited 2019 March 25] Available at: https://urn.nsk.hr/urn:nbn:hr:171:674539

M. Bošnjak, "KVANTITATIVNO ODREĐIVANJE SINTETSKIH KATINONA", Master's thesis, University of Split, School of Medicine, Split, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:171:674539

Metadata
TitleKVANTITATIVNO ODREĐIVANJE SINTETSKIH KATINONA
Title (english)QUANTITATIVE DETERMINATION OF SYNTHETIC CATHINONES
AuthorMartina Bošnjak
MentorDavorka Sutlović (mentor)
MentorMaja Veršić Bratinčević (komentor)
Committee memberMarija Definis-Gojanović (predsjednik povjerenstva)
Committee memberBranka Polić (član povjerenstva)
Committee memberDavorka Sutlović (član povjerenstva)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2018, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Ciljevi istraživanja: Razviti metodu za simultano određivanje koncentracija pet sintetskih katinona (bufedrona, mefedrona, DMMC-a, pentilona i MDPV-a) u mokraći, koja podrazumijeva izradu umjernih krivulja te njihovu primjenu na uzorcima u kojima su koncentracije nepoznate. Materijal i metode: Otopine sintetskih katinona za izradu umjerne krivulje dobivene su serijskim razrjeđivanjem referentnih standarda u mokraći te su zajedno ekstrahirane i pripremljene za analizu. Koncentracije bufedrona, mefedrona, DMMC-a, pentilona i MDPV-a određivale su se u dvama uzorcima mokraće u koje su dodani različiti nepoznati volumeni pripravljenih standardnih otopina. Ekstrahirani su metodom tekuće-tekuće (LLE), razrijeđeni kloroformom i analizirani tehnikom GC-MS snimanjem ukupne ionske struje (TIC). Rezultati: Kvantitativnom analizom sintetskih katinona pri 4000 ng/mL dobiveni su podaci o njihovim karakterističnim retencijskim vremenima (RT) i spektrima masa. Umjerne krivulje za simultano dokazivanje spomenutih sintetskih katinona uspješno su konstruirane pomoću dobivenih signala ciljanim mjerenjem otopina standarda u mokraći pri koncentracijama 200-4000 ng/mL. U oba biološka uzorka detektirani su svi sintetski katinoni te su očitane njihove nepoznate koncentracije. Prisutnost bufedrona, mefedrona, DMMC-a, pentilona i MDPV-a u oba uzorka mokraće potvrđena je i nakon derivatizacije. Zaključci: Razvijena je i provjerena GC-MS metoda primjenjiva za simultanu kvalitativnu i kvantitativnu analizu bufedrona, mefedrona, DMMC-a, pentilona i MDPV-a u biološkim uzorcima mokraće. To ima veliku važnost jer se sintetski katinoni brzo šire na globalnom tržištu. Daljnja istraživanja trebala bi uključiti veći broj sintetskih katinona i više vrsta bioloških uzoraka za dokazivanje njihovog prisutstva.
Abstract (english)
Objectives: The objective was to develop a method for simultaneous concentration determination of five synthetic cathinones in urine (buphedrone, mephedrone, DMMC, pentylone and MDPV), which implies plotting calibration curves and applying them to determine unknown concentrations in urine samples. Material and methods: In order to make the solutions of chosen synthetic cathinones used for creating calibration curves, serial dilution of reference standard solutions was made in a blank urine sample. Synthetic cathinones were extracted jointly and prepared for analysis. After adding different unknown volumes of standard solutions to two urine samples, unknown concentrations were determined. Liquid-liquid technique was used for the extraction of samples, followed by dilution in chloroform to prepare for a GC-MS analysis. The chosen working mode was total ion current (TIC). Results: Information about retention times (RT) and mass spectra of synthetic cathinones was obtained by qualitative analysis of concentration 4000 ng/mL. Calibration curves were successfully constructed using peak signals obtained by analysing concentrations 200-4000 ng/mL. In both urine samples all synthetic cathinones were detected and their unknown concentrations were determined. Their presence in both urine samples was additionally confirmed after derivatization. Conclusion: A GC-MS method applicable to simultaneous qualitative and quantitative analysis of buphedrone, mephedrone, DMMC, pentylone and MDPV in urine samples has been developed and tested. Such method development is valuable because synthetic cathinones are spreading rapidly across the global market. Further investigations ought to include more synthetic cathinones, as well as different types of biological samples.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:674539
Study programmeTitle: Farmacija
Study programme type: sveučilišni
Study level: integrirani preddiplomski i diplomski
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourcetext
File originBorn digital
Access conditionsOpen access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
Created on2019-01-24 13:37:46