The cytotoxic effect and apoptosis rate of breast cancer stem cells treated with the novel thieno[2,3-b]pyridine anticancer compound

Willmen, Louisa

master's thesis

Louisa Willmen (2019)

University of Split
School of Medicine
Medical chemistry and biochemistry

Cite this document...

APA 6th Edition

Willmen, L. (2019). The cytotoxic effect and apoptosis rate of breast cancer stem cells treated with the novel thieno[2,3-b]pyridine anticancer compound (Master's thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:264930

MLA 8th Edition

Willmen, Louisa. "The cytotoxic effect and apoptosis rate of breast cancer stem cells treated with the novel thieno[2,3-b]pyridine anticancer compound." Master's thesis, University of Split, School of Medicine, 2019. https://urn.nsk.hr/urn:nbn:hr:171:264930

Chicago 17th Edition

Harvard

Willmen, L. (2019). 'The cytotoxic effect and apoptosis rate of breast cancer stem cells treated with the novel thieno[2,3-b]pyridine anticancer compound', Master's thesis, University of Split, School of Medicine, accessed 19 August 2019, https://urn.nsk.hr/urn:nbn:hr:171:264930

Vancouver

Willmen L. The cytotoxic effect and apoptosis rate of breast cancer stem cells treated with the novel thieno[2,3-b]pyridine anticancer compound [Master's thesis]. Split: University of Split, School of Medicine; 2019 [cited 2019 August 19] Available at: https://urn.nsk.hr/urn:nbn:hr:171:264930

IEEE

L. Willmen, "The cytotoxic effect and apoptosis rate of breast cancer stem cells treated with the novel thieno[2,3-b]pyridine anticancer compound", Master's thesis, University of Split, School of Medicine, Split, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:171:264930

Cite this item: https://urn.nsk.hr/urn:nbn:hr:171:264930

Metadata

Title	The cytotoxic effect and apoptosis rate of breast cancer stem cells treated with the novel thieno[2,3-b]pyridine anticancer compound
Title (croatian)	Citotoksični učinak i apoptoza matičnih stanica karcinoma dojke tretiranih novosintetiziranim protutumorskim spojem tieno[2,3-b]piridinom
Author	Louisa Willmen
Mentor	Vedrana Čikeš Čulić (mentor)
Committee member	Anita Markotić (predsjednik povjerenstva)
Committee member	Ivana Mudnić (član povjerenstva)
Committee member	Ivana Pavlinac Dodig (član povjerenstva)
Granter	University of Split School of Medicine (Medical chemistry and biochemistry) Split
Defense date and country	2019-07-15, Croatia
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Basic Medical Sciences Medical Biochemistry
Abstract	Objectives: The purpose of this study is to determine the effects of treating breast cancer stem cells with the newly synthesized thieno[2,3-b]pyridine anticancer agent, by focusing on its cytotoxic and apoptotic effects on the investigated human cell line MDA-MB-231. Methods: The MDA-MB-231 triple-negative breast cancer cell line was treated with a newly developed thienopyridine anticancer compound (3-amino-N-(3-chloro-2-methylphenyl)-5- oxo-5,6,7,8-tetrahydrothieno[2,3-b]quinoline-2-carboxamide, 1) to determine its cytotoxic effect on triple negative breast cancer, the type of cell death it causes, and the cancer stem cell percentage after treatment. The 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay was performed, to analyze the cellular metabolic activity and determine the cytotoxic effect. Flow cytometric analysis was used in combination with Annexin-V-FITC and propidium iodide staining to assess the type of cell death after 48h of treatment with compound 1 (2 µM). Furthermore, flow cytometry also provided the percentage of CD44+/CD24- cancer stem cells after treatment. Results: Compound 1 was cytotoxic for breast cancer cells in a dose- and time-dependent manner; cell death occurs mainly by apoptosis. The percent of cancer stem cells decreased four times. Conclusion: Due to its cytotoxic effect on the percentage of triple negative breast cancer stem cells compound 1 can be a potential treatment for triple-negative breast cancer.
Abstract (croatian)	Ciljevi: Cilj ovog istraživanja je utvrditi učinke tretmana matičnih stanica karcinoma dojke novosintetiziranim protutumorskim spojem tieno [2,3-b] piridinom, s naglaskom na njegove citotoksične i apoptotske učinke na ispitivanu staničnu liniju MDA-MB-231. Materijali i metode: Trostruko negativna stanična linija karcinoma dojke MDA-MB-231 tretirana je novosintetiziranim tieno-piridinskim spojem (3-amino-N-(3-kloro-2-metilfenil)-5-okso-5,6,7,8-tetrahidrotieno[2,3-b]kinolin-2-karboksamid, 1) kako bi se utvrdio citotoksični učinak, tip stanične smrti i postotak matičnih stanica karcinoma nakon tretmana. Napravljen je MTT (3-(4,5-dimetiltiazolil-2)-2,5-difeniltetrazolij bromid) test kako bi se analizirala stanična metabolička aktivnost i utvrdio citotoksični učinak. Protočna citometrija u kombinaciji s bojanjem aneksin-V-FITC-om i propidij jodidom, korištena je kako bi se utvrdio tip stanične smrti nakon 48h tretmana spojem 1 (2 µM). Nadalje, protočnom citometrijom je također utvrđen postotak CD44+/CD24- matičnih stanica karcinoma nakon tretmana. Rezultati: Spoj 1 bio je citotoksičan za stanice karcinoma dojke te citotoksičnost korelira s koncentracijom spoja i vremenom inkubacije. Stanična smrt je nastupila prvenstveno zbog apoptoze. Postotak matičnih stanica karcinoma smanjio se četiri puta. Zaključci: Zbog citotoksičnog učinka na postotak matičnih stanica trostruko negativnog karcinoma dojke, spoj 1 može biti potencijalna terapija za trostruko negativni karcinom dojke.
Keywords
Keywords (croatian)
Language	english
URN:NBN	urn:nbn:hr:171:264930
Study programme	Title: Medicina na engleskom jeziku Study programme type: sveučilišni Study level: integrirani preddiplomski i diplomski Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Created on	2019-07-18 08:49:45