Background: The aim of this randomized study was to examine effects of subcutaneous instillation of local anesthetics at trocar sites combined with intraperitoneal analgesia, on postoperative pain among pediatric patients undergoing laparoscopic varicocelectomy. Methods: A total of 60 adolescents who underwent laparoscopic varicocelectomy, with median age of 16 (range 13-17) years were included in study. Based on type of local analgesic used during surgery, patients were randomly assigned in three study groups (20 patients in each group). The patients from Group I received 2% lidocaine, while the patients from Group II received 0.5% levobupivacaine. The patients from Group III (control group) did not receive any local anaesthetic intraoperatively. For each patient pain intensity was recorded according to the visual analogue scale (VAS) by a blinded investigator at 2, 6, 12 and 24 hours after the surgery. The primary outcome of the study were the effects of local anaesthetic administration on character and duration of postoperative pain. Secondary outcomes were postoperative complications and length of hospital stay. Results: Using GLM repeated measure model a significant effect of time (P=0.001) as well as interaction between time and study groups (P<0.001) on the intensity of pain, e.g. VAS score was detected. Furthermore, in regards to distinction between the study groups, a significant difference was found among the groups. For every time point the administration of 0.5% levobupivacaine resulted with significantly lower VAS pain score compared to administration of 2% lidocaine and control study group (P<0.001). In comparison with the control group, patients who received 2% lidocaine reported lower levels of pain according to VAS, except for time point at 24 hours after surgery. In regards to postoperative pain control, a significant difference was found among the groups. The patients who received any local anesthetic intraoperatively (n=5; 12.5%;) required significantly less analgesic than the patients from the control group (n=13; 65%) (P<0.001). Conclusion: A significant reduction in postoperative pain intensity and analgesics consumption in patients with laparoscopic varicocelectomy who received intraoperative local anesthetic at the skin incision sites and intraperitoneally was observed. The best effect was achieved when 0.5% levobupivacaine was administered.