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master's thesis
Utjecaj tienopiridinskog derivata na izražaj CD15s epitelnih i mezenhimalnih stanica karcinoma dojke
2021. urn:nbn:hr:171:852945
Vučenović, Nikolina
University of Split
School of Medicine
Medical chemistry and biochemistry

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Vučenović, N. (2021). Utjecaj tienopiridinskog derivata na izražaj CD15s epitelnih i mezenhimalnih stanica karcinoma dojke (Master's thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:852945

Vučenović, Nikolina. "Utjecaj tienopiridinskog derivata na izražaj CD15s epitelnih i mezenhimalnih stanica karcinoma dojke." Master's thesis, University of Split, School of Medicine, 2021. https://urn.nsk.hr/urn:nbn:hr:171:852945

Vučenović, Nikolina. "Utjecaj tienopiridinskog derivata na izražaj CD15s epitelnih i mezenhimalnih stanica karcinoma dojke." Master's thesis, University of Split, School of Medicine, 2021. https://urn.nsk.hr/urn:nbn:hr:171:852945

Vučenović, N. (2021). 'Utjecaj tienopiridinskog derivata na izražaj CD15s epitelnih i mezenhimalnih stanica karcinoma dojke', Master's thesis, University of Split, School of Medicine, accessed 16 April 2024, https://urn.nsk.hr/urn:nbn:hr:171:852945

Vučenović N. Utjecaj tienopiridinskog derivata na izražaj CD15s epitelnih i mezenhimalnih stanica karcinoma dojke [Master's thesis]. Split: University of Split, School of Medicine; 2021 [cited 2024 April 16] Available at: https://urn.nsk.hr/urn:nbn:hr:171:852945

N. Vučenović, "Utjecaj tienopiridinskog derivata na izražaj CD15s epitelnih i mezenhimalnih stanica karcinoma dojke", Master's thesis, University of Split, School of Medicine, Split, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:171:852945

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Metadata
TitleUtjecaj tienopiridinskog derivata na izražaj CD15s epitelnih i mezenhimalnih stanica karcinoma dojke
Title (english)The effect of thienopyridine derivate on CD15s expression in epithelial and mesenchymal cancer cells
AuthorNikolina Vučenović
MentorAnita Markotić (mentor)
Committee memberVedrana Čikeš Čulić (predsjednik povjerenstva)
Committee memberNikolina Režić Mužinić (član povjerenstva)
Committee memberMarino Vilović (član povjerenstva)
GranterUniversity of Split
School of Medicine
(Medical chemistry and biochemistry)
Split
Defense date and country2021, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract
CILJEVI ISTRAŽIVANJA: Ciljevi ovog istraživanja bili su odrediti izražaj CD15s na epitelnim (CD44-CD24+ i CD44-CD24-) i mezenhimalnim (CD44+CD24- tj. CSC) stanicama MDA-MB-231 stanične linije trostruko negativnog karcinoma dojke te odrediti izražaj CD44 kod CSC CD15s+ i CSC CD15s- stanica nakon primjene tienopiridinskog derivata. MATERIJALI I METODE: Novosintetizirani tienopiridinski derivat, 3-amino-N- (3-kloro-2-metilfenil)- 5 -okso -5,6,7,8-tetrahidrotieno [2,3-b] kinolin- 2-karboksamid, primijenjen je u 2 μM koncentraciji u trajanju od 48 sati na MDA-MB-231 staničnoj liniji. Potom su stanice imunobojene s anti-CD15s odnosno sekundarnim antitijelom na anti-CD15s konjugiranim s eFluor 660 fluorokromom, s anti-CD44-FITC i anti-CD24-PE te je fluorescencija pojedinih kromogena konačno mjerena protočnim citometrom. REZULTATI: U izražaju CD15s u CSC subpopulaciji nije bilo značajne razlike prije i nakon tretmana tienopiridinskim derivatom. Za razliku od CSC subpopulacije, ovaj je tretman rezultirao gotovo dvostrukim smanjenjem izražaja CD15s u subpopulaciji CD44- (P=0,004). Tretman nije utjecao na ekspresiju CD44 u CD15s+ CSC, ali je smanjio izražaj CD44 (P=0,008) u CD15s-CSC. ZAKLJUČAK: Nalazi sniženog izražaja CD15s kod epitelnih stanica i CD44 kod CD15- mezenhimalnih stanica ukazuju na potencijal novosintetiziranog tienopiridinskog derivata kao terapijskog sredstva za trostruko negativni rak dojke.
Abstract (english)
Objectives: The aims of this study were to analyse the expression of CD15s in epithelial (CD44-CD24+) and mesenchymal (CD44+CD24-, CSC) cells in MDA-MB-231 triple-negative breast cancer cell line and to measure expression of CD44 in CSC CD15s+ and CSC CD15s- cells after being treated with thieno[2,3-b]pyridine derivative. Materials and methods: Newly synthesized thieno[2,3-b]pyridine derivative of 3-amino-N-(3-chloro-2-methylphenyl)-5-oxo-5,6,7,8-tetrahydrothieno[2,3-b]quinoline-2-carboxamide was used in 2 μM concentration for 48 hours on MDA-MB-231 cell line. Thereafter, the cells were stained with anti-CD15s secondary antibody conjugated with eFluor 660 on CD15s fluorochrome, anti-CD44-FITC, anti-CD24-PE, and then chromogen fluorescence was analyzed by flow cytometry. Results: There was no difference in expression of CD15s in CSC subpopulation before and after treatment with thieno[2,3-b]pyridine derivative. Unlike CSC subpopulation, this treatment has resulted in almost two times reduction of CD15 expression in CD44- subpopulation (P=0.004). The treatment didn’t have any influence on the expression of CD44 in CD15s+CSC but it had reduced expression of CD44 (P=0.008) in CD15-CSC. Conclusion: The finding of reduced expression of CD15s in epithelial cells and CD44- within mesenchymal cells show the potential of newly synthesized thieno[2,3-b]pyridine derivative as a potential treatment option for triple-negative breast cancer.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:852945
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-07-21 07:50:22