prikaz prve stranice dokumenta Cytotoxic effect of thieno[2,3-b]pyridine derivatives on human bladder cancer cells
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master's thesis
Cytotoxic effect of thieno[2,3-b]pyridine derivatives on human bladder cancer cells
2022. urn:nbn:hr:171:938215
Kalezić, Denis
University of Split
School of Medicine

Cite this document

Kalezić, D. (2022). Cytotoxic effect of thieno[2,3-b]pyridine derivatives on human bladder cancer cells (Master's thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:938215

Kalezić, Denis. "Cytotoxic effect of thieno[2,3-b]pyridine derivatives on human bladder cancer cells." Master's thesis, University of Split, School of Medicine, 2022. https://urn.nsk.hr/urn:nbn:hr:171:938215

Kalezić, Denis. "Cytotoxic effect of thieno[2,3-b]pyridine derivatives on human bladder cancer cells." Master's thesis, University of Split, School of Medicine, 2022. https://urn.nsk.hr/urn:nbn:hr:171:938215

Kalezić, D. (2022). 'Cytotoxic effect of thieno[2,3-b]pyridine derivatives on human bladder cancer cells', Master's thesis, University of Split, School of Medicine, accessed 12 November 2024, https://urn.nsk.hr/urn:nbn:hr:171:938215

Kalezić D. Cytotoxic effect of thieno[2,3-b]pyridine derivatives on human bladder cancer cells [Master's thesis]. Split: University of Split, School of Medicine; 2022 [cited 2024 November 12] Available at: https://urn.nsk.hr/urn:nbn:hr:171:938215

D. Kalezić, "Cytotoxic effect of thieno[2,3-b]pyridine derivatives on human bladder cancer cells", Master's thesis, University of Split, School of Medicine, Split, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:171:938215

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Metadata
TitleCytotoxic effect of thieno[2,3-b]pyridine derivatives on human bladder cancer cells
Title (croatian)Citotoksično djelovanje tieno[2,3-b]piridinskih spojeva na stanice humanog karcinoma mokraćnog mjehura
AuthorDenis Kalezić
MentorVedrana Čikeš Čulić (mentor)
Committee memberIvana Novak Nakir (predsjednik povjerenstva)
Committee memberNikolina Režić Mužinić (član povjerenstva)
Committee memberIvana Pavlinac Dodig (član povjerenstva)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2022-09-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract
Objectives: The purpose of this study was to determine the effects of treating bladder cancer cells with the newly synthesized thieno[2,3-b]pyridine anticancer agents, by focusing on its cytotoxic effects on the investigated human cell line T24. Methods: The T24 bladder cancer cell line was treated with a newly developed thieno[2,3-b]pyridine anticancer compounds: Inhibitor 5 3-amino-N-(3-chloro-2-methylphenyl)-5-oxo-5,6,7,8-tetrahydrothieno[2,3-b]quinoline-2-carboxamide, Inhibitor 6 3-amino-N-(naphthalen-1-yl)-5-oxo-5,6,7,8-tetrahydrothieno[2,3-b]quinoline-2-carboxamide, Inhibitor 8 (E)-3-amino-5-(3-(3-bromophenyl)acryloyl)-N-(3-chloro-2-methylphenyl)-6-methylthieno[2,3-b]pyridine-2-carboxamide and Inhibitor 9 (E)-3-amino-5-(3-(3-bromophenyl)-1-hydroxyallyl)-N-(3-chloro-2-methylphenyl)-6-methylthieno[2,3-b]pyridine-2-carboxamide to determine its cytotoxic effect on T24 cells. The 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to analyze the cellular metabolic activity and determine the cytotoxic effect. Results: Inhibitors 5, 6, 8 and 9 all showed significant cytotoxic effect on the bladder cancer cells in a dose- and time-dependent manner. The most effective was Inhibitor 8 with an IC50 value of 0.4041 μg/mL after 72 hours. Conclusion: Due to their cytotoxic effect on bladder cancer cells, Inhibitors 5, 6, 8 and 9 deserve further attention as a potential treatment for transitional cell cancer.
Abstract (croatian)
Ciljevi: Svrha ove studije bila je utvrditi učinke tretiranja stanica raka mokraćnog mjehura novosintetiziranim tieno[2,3-b]piridin antitumorskim spojevima, fokusirajući se na njihove citotoksične učinke na ispitivanu humanu staničnu liniju T24. Materijali i metode: T24 stanična linija raka mokraćnog mjehura tretirana je novosintetiziranim tieno[2,3-b]piridin antitumorskim spojevima: Inhibitor 5 - 3-amino-N-(3-kloro-2-metilfenil)-5-okso-5,6,7,8-tetrahidrotieno[2,3-b]kinolin-2-karboksamid, Inhibitor 6 - 3-amino-N-(naftalen-1-il)-5-okso-5,6,7,8-tetrahidrotieno[2,3 -b]kinolin-2-karboksamid, Inhibitor 8 - (E)-3-amino-5-(3-(3-bromofenil)akriloil)-N-(3-klor-2-metilfenil)-6-metiltieno[2, 3-b]piridin-2-karboksamid i Inhibitor 9 - (E)-3-amino-5-(3-(3-bromofenil)-1-hidroksialil)-N-(3-klor-2-metilfenil)-6- metiltieno[2,3-b]piridin-2-karboksamid, kako bi se odredio njihov citotoksični učinak na T24 stanice. Proveden je test 3-(4,5-dimetiltiazolil-2)-2,5-difeniltetrazolij bromida (MTT) kako bi se analizirala stanična metabolička aktivnost i odredio citotoksični učinak. Rezultati: Svi inhibitori 5, 6, 8 i 9 pokazali su značajan citotoksični učinak na stanice raka mokraćnog mjehura i taj učinak je ovisan o dozi i vremenu inkubacije. Najučinkovitiji je bio Inhibitor 8 s IC50 vrijednošću od 0,4041 μg/mL nakon 72 sata. Zaključci: Zbog svog citotoksičnog učinka na stanice raka mokraćnog mjehura, Inhibitori 5, 6, 8 i 9 zaslužuju daljnju pozornost kao potencijalni tretman za rak prijelaznih stanica.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:171:938215
Study programmeTitle: Medical Studies in English
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-09-15 11:39:14