Objective: Understanding student attitudes toward smoking and the use of tobacco products is important for effective smoking prevention interventions. In this study the prevalence of the use of tobacco products among students at the University of Split was assessed, as well as their knowledge, attitudes, and perceptions regarding the harmful effects of tobacco products. Materials and methods: Cross-sectional research was conducted using an online questionnaire. A total of 1184 students aged ≥ 18 years who were full-time students in the winter semester of 2022 at the University of Split participated. The obtained data were analyzed using the Mann-Whitney U and Kruskal-Wallis tests, as well as regression analysis, which checked the connection between the subjects' knowledge and the variables of interest. Results: Among the surveyed students, 30.2% were smokers, 74.5% of which smoked classic cigarettes, and 17.6% smoked tobacco products. The average knowledge of all respondents was 16.2±7.1 (range from 0 to 27 correct answers). Better results in knowledge were shown by older students, higher years of study, students studying biomedicine and healthcare, and those who notice health warnings on cigarette packages and believe that smoking should be prohibited in public indoor spaces (P≤0.001). Only 29.0% of students knew that a cigarette burns at 800 degrees. Also, 37.2% knew that exposure to passive smoking is associated with cognitive deficits, and 38.9 % of them knew that smoking lowers bone density. Conclusions: The results of the research confirmed the gaps in knowledge and misconceptions regarding the harms related to the use of tobacco products. The findings highlight the need for clearer promotion of prevention and raising awareness of the harmful effects of smoking on human health.