Introduction: Interprofessional collaboration between physicians and pharmacists has been recognized as a one of the potential solutions for early detection of chronic diseases, reduction of medication errors and increase in patients’ adherence. The requirements for such collaboration are positive attitudes in both professions. Objective: The first aim of this study was to determine the attitudes towards interprofessional collaboration between physicians and pharmacists in primary health care, as well as between medical and pharmacy students in Croatia. The second aim was to determine whether the participation in the interprofessional pharmacotherapy workshop could positively affect their attitudes. Materials and Methods: Cross-sectional studies were conducted among physicians (N=81) and pharmacists (N=432) in 9 Croatian cities (Zagreb, Split, Zadar, Dubrovnik, Rijeka, Osijek, Slavonski Brod, Varaždin i Čakovec) and among medical (N=258) and pharmacy (N=107) students at the University of Split School of Medicine. Furthermore, two interprofessional pharmacotherapy workshops were organized - the first one for medical (N=42) and pharmacy students (N=38) and the second one for physicians (N=18) and pharmacists (N=23). Attitudes towards interprofessional collaboration between pharmacists and physicians were accessed before and after each workshop. Results: The medical students have shown 24 % less positive attitudes than pharmacy students and were the group with the least positive attitudes towards physicians and pharmacist collaboration. Physicians have shown less positive attitudes than pharmacist towards their collaboration, but the difference in their attitudes was only 6 %. Participation in the interprofessional pharmacotherapy workshop significantly increased the attitudes of physicians, medical and pharmacy students, while the pharmacists’ attitudes have not been changed. Conclusion: The moderately large difference between medical and pharmacy students’ attitudes towards interprofessional collaboration, could have a negative impact on their future collaboration. Interprofessional pharmacotherapy workshops have proved to be a successful model of interprofessional education in reducing these differences and approaching their attitudes, as well as attitudes of physicians and pharmacists.