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doctoral thesis
Izražaj SATB1 i PTEN-a u bubrezima dijabetičkih štakora tijekom starenja
2018. urn:nbn:hr:171:675925
Delić Jukić, Ivana Kristina
University of Split
School of Medicine

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Delić Jukić, I. K. (2018). Izražaj SATB1 i PTEN-a u bubrezima dijabetičkih štakora tijekom starenja (Doctoral thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:675925

Delić Jukić, Ivana Kristina. "Izražaj SATB1 i PTEN-a u bubrezima dijabetičkih štakora tijekom starenja." Doctoral thesis, University of Split, School of Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:171:675925

Delić Jukić, Ivana Kristina. "Izražaj SATB1 i PTEN-a u bubrezima dijabetičkih štakora tijekom starenja." Doctoral thesis, University of Split, School of Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:171:675925

Delić Jukić, I. K. (2018). 'Izražaj SATB1 i PTEN-a u bubrezima dijabetičkih štakora tijekom starenja', Doctoral thesis, University of Split, School of Medicine, accessed 04 December 2024, https://urn.nsk.hr/urn:nbn:hr:171:675925

Delić Jukić IK. Izražaj SATB1 i PTEN-a u bubrezima dijabetičkih štakora tijekom starenja [Doctoral thesis]. Split: University of Split, School of Medicine; 2018 [cited 2024 December 04] Available at: https://urn.nsk.hr/urn:nbn:hr:171:675925

I. K. Delić Jukić, "Izražaj SATB1 i PTEN-a u bubrezima dijabetičkih štakora tijekom starenja", Doctoral thesis, University of Split, School of Medicine, Split, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:171:675925

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Metadata
TitleIzražaj SATB1 i PTEN-a u bubrezima dijabetičkih štakora tijekom starenja
Title (english)Changes in SATB1 and PTEN expression in the kidneys of diabetic rats during ageing
AuthorIvana Kristina Delić Jukić https://orcid.org/0000-0002-0738-7792
MentorKatarina Vukojević (mentor)
Committee memberDragan Ljutić (predsjednik povjerenstva)
Committee memberInes Drenjančević (član povjerenstva)
Committee memberTatijana Zemunik (član povjerenstva)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2018, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Cytology, Histology and Embryology
Universal decimal classification
(UDC)		611 - Anatomy
Abstract
Dijabetička bolest bubrega je uobičajena komplikacija šećerne bolesti. Opisujemo izražaj tkivno specifične jezgrine matriks vezajuće bjelančevine SATB1 i fosfataza i tenzinskog homologa PTEN u bubrezima dijabetičkih štakora tijekom starenja. Mužjaci štakora Sprague Dawley su dobili injekcije streptozotocina 55mg/kg (STZ) (skupina štakora sa šećernom bolesti) ili citratni pufer (kontrolna skupina). Nakon 2 tjedna, 6 i 12 mjeseci analizirano je tkivo bubrega u tri različita područja: glomeruli, proksimalni i distalni tubuli (PT i DT). Tkivo je obojano imunohistokemijski sa SATB1 i PTEN. Nakon 2 tjedna dijabetički štakori su imali veći izražaj SATB1, a manji izražaj PTEN-a. Nakon 6 i 12 mjeseci PTEN je bio više izražen u kontrolnim skupinama. Nakon 12 mjeseci SATB1 je bio više izražen u dijabetičkih štakora. U glomerulima, PTEN je bio više izražen u kontrolnim skupinama štakora, a kod dijabetičkih štakora se bilježi veći izražaj SATB1, ali tek nakon 12 mjeseci. Izražaj PTEN-a se povećavao od 2 tjedna do 12 mjeseci u proksimalnim i distalnim tubulima kontrolnih skupina. SATB1 je bio izražen isključivo u proksimalnim tubulima dijabetičkih štakora nakon 2 tjedna, a u distalnim tubulima se prati veći izražaj SATB1 u kontrolnim skupinama. Nakon 6 mjeseci u proksimalnim i distalnim tubulima je bio veći izražaj SATB1 kod dijabetičkih štakora. Glavne promjene u izražaju oba biljega događaju se već nakon 2 tjedna utjecaja šećerne bolesti, osobito u proksimalnim tubulima, što ukazuje na rani početak patofizioloških promjena u dijabetičkim bubrezima, što bi se normalno događalo starenjem. Ova otkrića mogu pridonijeti našem razumijevanju dijabetičke bolesti bubrega i mogućim pravim ciljevima liječenja.
Abstract (english)
Diabetic nephropathy (DN) is a common complication of diabetes mellitus (DM). We describe special AT-rich sequence binding protein 1 (SATB1) and phosphatase and tensin homologue (PTEN) expression in the kidneys of diabetic rats during ageing. Male Sprague Dawley rats were injected with 55mg/kg streptozotocin (STZ) (DM group) or with citrate buffer (control). Kidneys were collected after 2 weeks, 6 months and 12 months and were analyzed in three different areas: glomeruli, proximal and distal convoluted tubules (PCT and DCT). The sections were stained immunohistochemically, using SATB1 and PTEN. Significant difference was observed after 2 weeks with more SATB1 expression and less PTEN expression in diabetic rats. PTEN was more expressed in controls after 6 and 12 months. After 12 months SATB1 was more expressed in diabetic rats. In glomeruli, control rats had more expression of PTEN and diabetic rats had more expression of SATB1 only after 12 months. The expression of PTEN was increasing from 2 weeks to 12 months in PCT and DCT of control rats. SATB1 was expressed exclusively in PCT of diabetic rats after 2 weeks, and expression in DCT was higher in controls. After 6 months PCT and DCT had more SATB1 expression in diabetic rats. The major changes in expression of both markers occur already after 2 weeks of DM, especially in PCT, implying the early onset of pathophysiological changes in diabetic kidneys, which would normally occur with ageing. These findings may contribute to our understanding of changes associated with DN and possible proper treatment modalities.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:675925
Study programmeTitle: Biology of Neoplasms
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-05-12 07:25:11