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master's thesis
Izražaj imunosupresijskih molekula PD-1 i CTLA-4 u zloćudnim tumorima mokraćnog mjehura
2015. urn:nbn:hr:171:224111
Čičmir-Vestić, Mate
University of Split
School of Medicine

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Čičmir Vestić, M. (2015). Izražaj imunosupresijskih molekula PD-1 i CTLA-4 u zloćudnim tumorima mokraćnog mjehura (Master's thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:224111

Čičmir Vestić, Mate. "Izražaj imunosupresijskih molekula PD-1 i CTLA-4 u zloćudnim tumorima mokraćnog mjehura." Master's thesis, University of Split, School of Medicine, 2015. https://urn.nsk.hr/urn:nbn:hr:171:224111

Čičmir Vestić, Mate. "Izražaj imunosupresijskih molekula PD-1 i CTLA-4 u zloćudnim tumorima mokraćnog mjehura." Master's thesis, University of Split, School of Medicine, 2015. https://urn.nsk.hr/urn:nbn:hr:171:224111

Čičmir Vestić, M. (2015). 'Izražaj imunosupresijskih molekula PD-1 i CTLA-4 u zloćudnim tumorima mokraćnog mjehura', Master's thesis, University of Split, School of Medicine, accessed 18 November 2024, https://urn.nsk.hr/urn:nbn:hr:171:224111

Čičmir Vestić M. Izražaj imunosupresijskih molekula PD-1 i CTLA-4 u zloćudnim tumorima mokraćnog mjehura [Master's thesis]. Split: University of Split, School of Medicine; 2015 [cited 2024 November 18] Available at: https://urn.nsk.hr/urn:nbn:hr:171:224111

M. Čičmir Vestić, "Izražaj imunosupresijskih molekula PD-1 i CTLA-4 u zloćudnim tumorima mokraćnog mjehura", Master's thesis, University of Split, School of Medicine, Split, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:171:224111

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Metadata
TitleIzražaj imunosupresijskih molekula PD-1 i CTLA-4 u zloćudnim tumorima mokraćnog mjehura
AuthorMate Čičmir-Vestić
MentorJanoš Terzić (mentor)
MBZ: 209906
GranterUniversity of Split
School of Medicine
Split
Defense date and country2015, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Urology
Abstract
Cilj istraživanja: Utvrditi postoji li povećan izražaj gena CTLA-4 i PD-1 u urotelnim tumorima mokraćnoga mjehura niskog gradusa. Metode: Iz uzoraka je izolirana molekula mRNA, nakon čega je RNA reverznom transkripcijom prevedena u cDNA koja je služila kao predložak za qRT-PCR. Elektroforezom na agaroznom gelu potvrđena je specifičnost početnica, a razina promjene izražaja gena utvrđena je usporedbom s izražajom gena RPS23. Rezultati: Gen za CTLA-4 ima pojačan izražaj u 10 od 12 urotelnih tumora mokraćnog mjehura niskog gradusa (P = 0,0433). Gen za PD-1 ima tedenciju k povećanom izražaju (u 7 od 12 tumora mokraćnog mjehura, P = 0,7728) Zaključci: Izražaj gena CTLA-4 statistički značajno je povećan u urotelnim tumorima mokraćnog mjehura niskog gradusa. Gen PD-1 nema statistički značajno povišen izražaj u urotelnim tumorima mokraćnog mjehura niskog gradusa, iako postoji tendencija njegovom povećanom izražaju. Rezultati ovog rada ukazuju na moguću korisnost inhibicije molekula CTLA-4 i PD-1 pri liječenju urotelnih tumora.
Abstract (english)
Aims: To determine level of expression of immunospressive CTLA-4 and PD-1 molecules in low grade urothelial tumors. Methods: RNA was isolated from tissues samples and then converted into cDNA by reverse transcription. cDNA was used as a template for qRT-PCR reaction. Specificity of the amplification reaction was verified by agarose gel electrophoresis. RPS23 was used as a housekeeping control. Results: CTLA-4 was over-expressed in 10 of 12 urothelial bladder tumors (P = 0,0433). PD-1 gene display tendency to elevated expression (7 of 12 urothelial bladder tumors showed overexpression, P = 0,7728) Conclusions: Expression of CTLA-4 is significantly elevated in low grade urothelial bladder tumors. PD-1 expression is not significant elevated although there is trend toward its elevation. These results imply great potential of inhibiting CTLA-4 and PD-1 molecules during urothelial cancer therapy.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:224111
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2016-02-08 14:20:57