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doctoral thesis
Uloga fibrinogena u idiopatskom sindromu karpalnog tunela
2014. urn:nbn:hr:171:433373
Utrobičić, Ivan
University of Split
School of Medicine

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Utrobičić, I. (2014). Uloga fibrinogena u idiopatskom sindromu karpalnog tunela (Doctoral thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:433373

Utrobičić, Ivan. "Uloga fibrinogena u idiopatskom sindromu karpalnog tunela." Doctoral thesis, University of Split, School of Medicine, 2014. https://urn.nsk.hr/urn:nbn:hr:171:433373

Utrobičić, Ivan. "Uloga fibrinogena u idiopatskom sindromu karpalnog tunela." Doctoral thesis, University of Split, School of Medicine, 2014. https://urn.nsk.hr/urn:nbn:hr:171:433373

Utrobičić, I. (2014). 'Uloga fibrinogena u idiopatskom sindromu karpalnog tunela', Doctoral thesis, University of Split, School of Medicine, accessed 14 November 2024, https://urn.nsk.hr/urn:nbn:hr:171:433373

Utrobičić I. Uloga fibrinogena u idiopatskom sindromu karpalnog tunela [Doctoral thesis]. Split: University of Split, School of Medicine; 2014 [cited 2024 November 14] Available at: https://urn.nsk.hr/urn:nbn:hr:171:433373

I. Utrobičić, "Uloga fibrinogena u idiopatskom sindromu karpalnog tunela", Doctoral thesis, University of Split, School of Medicine, Split, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:171:433373

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Metadata
TitleUloga fibrinogena u idiopatskom sindromu karpalnog tunela
Title (english)The role of fibrinogen in idiopathic carpal tunnel syndrome
AuthorIvan Utrobičić
MentorJanoš Terzić (mentor)
Committee memberValdi Pešutić-Pisac (predsjednik povjerenstva)
Committee memberSnježana Tomić (član povjerenstva)
Committee memberVladimir Boschi (član povjerenstva)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2014, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Neurology
Universal decimal classification
(UDC)		616 - Pathology. Clinical medicine
Abstract
UVOD. Idiopatski sindrom karpalnog tunela (ICTS) je najučestalija kompresijska neuropatija koja češće zahvaća žene. Iako su neki slučajevi ICTS-a povezani s mutacijama u genu za transtiretin ili su udruženi sa sistemskom amiloidozom, glavnina slučajeva sindroma karpalnog tunela je nepoznate etiologije. METODE. U studiju je uključen 71 pacijent s ICTS-om i 68 kontrolnih ispitanika. Razina fibrinogena u plazmi mjerena je prije operacije, a depoziti fibrinogena u transverzalnom karpalnom ligamentu (TCL) su pronađeni imunohistokemijskom metodom, te potvrđeni western blot-om i masenom spektrometrijom. Interakcija fibrinogena s drugim proteinima je proučavana imunoprecipitacijskim esejom. REZULTATI: Pokazali smo da je povišena razina proupalnog i hemostatskog proteina fibrinogena u plazmi bolesnika s ICTS-om. Mjerenja drugih sistemskih upalnih biljega nisu pokazala odstupanja od normale, a lokalni upalni odgovor u TCL-u bio je odsutan. Bolesnici s ICTS-om imali su kraće vrijeme krvarenja, najvjerojatnije radi povećane razine fibrinogena u plazmi. Poznato je polimorfizam u promotorskoj regiji gena za fibrinogen B povezan s povišenom razinom fibrinogena u plazmi, ali takva povezanost nije primijećena kod bolesnika s ICTS-om. Iako smo ustanovili da je transkripcijska aktivnost gena za fibrinogen u TCL-u niska, otkrili smo depozite fibrinogena u TCL-u. Neki proteini koji se odlažu kod neurodegenerativnih bolesti, uključujući transtitetin i α-synuclein također su pronađeni u TCL-u te je njihova lokalna transkripcijska aktivnost visoka. Naposljetku, pokazali smo da je fibrinogen u interakciji s transtiretinom i α-synucleinom u lizatima tkiva TCL-a. ZAKLJUČAK: Podatci u ovoj studiji pokazuju da fibrinogen i drugi proteini skloni agregaciji imaju važnu ulogu u patogenezi ICTS-a
Abstract (english)
OBJECTIVE. Idiopathic carpal tunnel syndrome (ICTS) is a common entrapment neuropathy that primarily affects women. Though some cases of ICTS are linked to mutations in the transthyretin gene or are associated with systemic amyloidosis, the majority of ICTS cases are of unknown etiology. METHODS. A total of 71 ICTS patients and 68 control individuals were included in the study. Fibrinogen levels were determined prior to surgery and fibrinogen deposition in transversal carpal ligament (TCL) was detected by immunohistochemistry, western blot and mass spectrometry. Fibrinogen interaction with other proteins was studied by immunoprecipitation assays. RESULTS. We report that plasma levels of the pro-inflammatory and haemostatic protein fibrinogen is elevated in ICTS patients. Other measured systemic inflammatory markers were not affected and local inflammatory responses in TCL were absent. ICTS patients have shorter bleeding times most probably due to elevated plasma levels of fibrinogen. Polymorphisms of the fibrinogen B promoter region were previously associated with elevated plasma fibrinogen but this association was not observed among patients with ICTS. Interestingly, we detected fibrinogen deposits in the TCL while transcriptional activity of the fibrinogen genes in TCL was low. Some amyloidogenic proteins, including transthyretin and -synuclein, were also found in the TCL while their local transcriptional activity was rather high. Finally, we demonstrated that fibrinogen interacts with transthyretin and -synuclein in TCL lysates. CONCLUSIONS. Our data indicate that fibrinogen and other aggregation-prone proteins potentially have an important role in the pathogenesis of ICTS.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:433373
Study programmeTitle: Basic and clinical medical sciences
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstva, polje kliničke medicinske znanosti (doktor/doktorica znanosti, područje biomedicine i zdravstva, polje kliničke medicinske znanosti)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2023-06-02 07:53:37