Objectives: To determine the knowledge and attitudes of students of the University of Split School of Medicine on ecopharmacovigilance and to examine the ways of storing and disposing of drugs, especially expired medicines. Subjects and methods: Students of medicine, dental medicine and pharmacy from the University of Split School of Medicine participated in the research in the academic year 2022/2023. Students' knowledge and habits were examined by a previously published survey. The survey contains twenty questions divided into three parts and the possibility of writing an additional note. Results: A total of 80 students of the University of Split School of Medicine participated in the study, of which 39 were medical students, 14 were dental students and 27 were pharmacy students. The largest proportion were female students, and only a sixth of the students were male. The knowledge score of students of different study program did not differ significantly (P = 0.533), as well as the knowledge score of students who have a family member in health care and those who do not (P = 0.254). Furthermore, 86.2% believed that there should be clear consumer guidance on the safe disposal of medicines. Pharmacy students mostly (51.9 %) educated their friends or family members about the safe disposal of medicines (P = 0.018). Also, some students, especially pharmacy students, responded that the responsibility for improving knowledge among households about the proper disposal of unused drugs lies with the pharmacist. Conclusion: The results of this study showed that there is no statistically significant difference in knowledge on ecopharmacovigilance among students of the University of Split School of Medicine with regard to the study program as well as knowledge between students who have family members in the healthcare sector and students who do not. It was observed that pharmacy students educated their friends or family members to the greatest extent about the safe disposal of medicines and that some students, especially pharmacy students, felt that it was the pharmacist's responsibility to improve knowledge among households about the proper disposal of unused medicines.