Title Procjena oštećenja bukalnih stanica usne šupljine uzrokovanih fluoridima Title (english) Assessment of damage to the buccal cells of the oral cavity caused by fluoride Author Ema Puizina Mladinić Mentor Antonija Tadin (mentor)Committee member Ivan Galić (predsjednik povjerenstva)Committee member Vedrana Čikeš Čulić (član povjerenstva)Committee member Daniel Jerković (član povjerenstva)Granter University of Split Defense date and country 2024-03-05, Croatia Scientific / art field, BIOMEDICINE AND HEALTHCARE Universal decimal classificationUDC ) 616.31 - Stomatology Abstract Ciljevi: Glavni cilj istraživanja je procijeniti potencijalni citotoksični i genotoksični učinak
kombiniranog korištenja fluoridiranih zubnih pasta i tekućine za ispiranje usne šupljine s
fluoridom mjerenjem pojavnosti mikronukleusa i ostalih jezgrinih anomalija u stanicama
bukalne sluznice mikronukleus testom. Sporedni ciljevi su procijeniti potencijalni citotoksični
i genotoksični učinak zubnih pasta s različitim koncentracija fluorida na pojavnost
mikronukleusa i ostalih jezgrinih
... More anomalija u stanicama bukalne sluznice mikronukleus testom
te ispitati znanja, stavove i ponašanje ispitanika prema oralnom zdravlju.
Materijali i metode: Ovo prospektivno, dvostruko slijepo, paralelno randomizirano kliničko
ispitivanje provedeno je pomoću analize bukalnog mikronukleusnog citoma (BMCyt analiza).
Četrdeset i jedan ispitanik nasumično je raspoređen u dvije skupine. Svi su ispitanici koristili
iste vrste pasta za zube 12 tjedana, dizajniranih izričito za ovu studiju (bez fluorida, 1050 ppm
NaF, odnosno 1450 ppm NaF; svaka kroz 4 tjedna). Istovremeno, tijekom tri mjeseca
istraživanja, jedna skupina koristila je tekućini za ispiranje usta s fluoridom (450 ppm NaF), a
druga bez fluorida. Uzorak bukalne sluznice uzet je prije uporabe testiranih proizvoda te nakon
4, 8 i 12 tjedana njihove uporabe. Anketni upitnik o znanjima, stavovima i ponašanju sadržavao
je 11 pitanja o znanju, 8 o stavovima i 7 o ponašanju prema oralnom zdravlju.
Rezultati: Učestalost mikronukleusa i većina drugih bodovanih parametara iz BMCyt testa nije
pokazala statistički značajne razlike unutar i između ispitivanih skupina. Uspoređujući dvije
skupine, samo je statistički značajno povećanje broja stanica s jezgrinim pupoljcima (p = 0,048)
i karioreksom (p = 0,020) nakon 4 tjedna korištenja primijećeno u skupini koja je koristila
tekućinu za ispiranje usta s fluoridom. Znanja, stavovi i ponašanje prema oralnom zdravlju
također ne pokazuju značajnija odstupanja od točnih odgovora i pozitivnog stava te ponašanja,
a za bolje riješen upitnik o znanjima, stavovima i ponašanju prema oralnom zdravlju značajni
negativni prediktori su iznadprosječni socio-ekonomski status (OR = 0,023; 95 % CI 0,0007 -
0,794; p = 0,036) i visoka stručna sprema (OR = 0,010; 95 % CI 0,0002 - 0,515; p = 0,021).
Zaključak: Na temelju rezultata može se zaključiti da istovremena primjena fluoridirane zubne
paste i fluoridirane tekućine za usta ne dovodi do citotoksičnog i genotoksičnog oštećenja
stanica bukalne sluznice. Na broj stanica s kariolizom utječe konzumacija mesa, a na broj
stanica s karioreksom utječe muški spol. Za bolje riješen upitnik o znanjima, stavovima i
ponašanju prema oralnom zdravlju značajni negativni prediktori su iznadprosječni socio -
ekonomski status i visoka stručna sprema. Less Abstract (english) Objectives: The main objective is to evaluate the potential cytotoxic and genotoxic effect of
the combined use of fluoridated toothpastes and mouthwashes with fluoride by measuring the
occurrence of micronuclei and other nuclear anomalies in the cells of the buccal mucosa using
the micronucleus test. Secondary objectives are to assess the potential cytotoxic and genotoxic
effect of toothpastes with different concentrations of fluoride on the occurrence of micronuclei
and other
... More nuclear anomalies in the cells of the buccal mucosa using the micronucleus test, and
to examine the knowledge, attitudes and behavior towards the oral health.
Materials and methods: This prospective, double-blinded parallel randomized clinical trial
was conducted using a buccal micronucleus cytome assay (BMCyt assay). Forty-one
participants were randomly assigned to the two groups. All participants have been using the
same kinds of toothpaste for 12 weeks, designed explicitly for this study (non-fluoride, 1050
ppm NaF, and 1450 ppm NaF each for four weeks, respectively). Simultaneously, during the
three months of the research, one group used mouthwash with fluoride (450 ppm NaF) and
another without fluoride. The buccal mucosal sampling was taken before using the tested
products and after 4, 8, and 12 weeks of their use. The survey questionnaire on knowledge,
attitudes and behavior had 11 questions on knowledge, 8 on attitudes and 7 on behavior towards
oral health.
Results: The frequency of micronuclei and the majority of other scored end-points from the
BMCyt assay showed no statistically significant differences within and between the studied
groups. Comparing two groups only statistically significant increase in the number of cells with
nuclear buds (p = 0,048) and karyorrhexis (p = 0,020) at four weeks of usage were observed in
the group that used mouthwash with fluoride. Knowledge, attitudes and behavior towards the
oral health also does not show significant deviations from correct answers and positive attitude
and behavior, and for the better-solved questionnaire on knowledge, attitudes and behavior
towards oral health, significant negative predictors are above-average socio-economic status
(OR = 0,023; 95 % CI 0,0007 - 0,794; p = 0,036) and higher education grade (OR = 0,010; 95
% CI 0,0002 - 0,515; p = 0,021).
Conclusion: Based on the results, it can be concluded that simultaneous application of
fluoridated toothpaste and fluoride mouthwash does not lead to cytotoxic or genotoxic damage in buccal mucosal cells. The number of cells with karyolysis is affected by meat consumption,
and the number of cells with karyorrhexis is affected by male gender. For the better-solved
questionnaire on knowledge, attitudes and behavior towards oral health, significant negative
predictors are above-average socio-economic status and high professional education. Less Keywords
usta
sluznica usta
zubni karijes
fluoridi
bukalni mikronukleusni citom test
Keywords (english)
Mouth
Mouth Mucosa
Dental Caries
Fluorides
Buccal Micronucleus Cytome assay
Language croatian URN:NBN urn:nbn:hr:171:933481 Promotion 2024 Study programme Title: Evidence-Based Clinical Medicine Type of resource Text File origin Born digital Access conditions Open access Terms of use Created on 2024-03-14 11:27:33
