prikaz prve stranice dokumenta Effect of prostaglandin F2 analog treatment on proliferation and MAPK/ERK signaling in conjunctival tissues of glaucoma patients
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master's thesis
Effect of prostaglandin F2 analog treatment on proliferation and MAPK/ERK signaling in conjunctival tissues of glaucoma patients
2024. urn:nbn:hr:171:693049
Kelava, Filip
University of Split
School of Medicine

Cite this document

Kelava, F. (2024). Effect of prostaglandin F2 analog treatment on proliferation and MAPK/ERK signaling in conjunctival tissues of glaucoma patients (Master's thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:693049

Kelava, Filip. "Effect of prostaglandin F2 analog treatment on proliferation and MAPK/ERK signaling in conjunctival tissues of glaucoma patients." Master's thesis, University of Split, School of Medicine, 2024. https://urn.nsk.hr/urn:nbn:hr:171:693049

Kelava, Filip. "Effect of prostaglandin F2 analog treatment on proliferation and MAPK/ERK signaling in conjunctival tissues of glaucoma patients." Master's thesis, University of Split, School of Medicine, 2024. https://urn.nsk.hr/urn:nbn:hr:171:693049

Kelava, F. (2024). 'Effect of prostaglandin F2 analog treatment on proliferation and MAPK/ERK signaling in conjunctival tissues of glaucoma patients', Master's thesis, University of Split, School of Medicine, accessed 27 January 2025, https://urn.nsk.hr/urn:nbn:hr:171:693049

Kelava F. Effect of prostaglandin F2 analog treatment on proliferation and MAPK/ERK signaling in conjunctival tissues of glaucoma patients [Master's thesis]. Split: University of Split, School of Medicine; 2024 [cited 2025 January 27] Available at: https://urn.nsk.hr/urn:nbn:hr:171:693049

F. Kelava, "Effect of prostaglandin F2 analog treatment on proliferation and MAPK/ERK signaling in conjunctival tissues of glaucoma patients", Master's thesis, University of Split, School of Medicine, Split, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:171:693049

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TitleEffect of prostaglandin F2 analog treatment on proliferation and MAPK/ERK signaling in conjunctival tissues of glaucoma patients
Title (croatian)Učinak liječenja analozima prostaglandina F2 na proliferaciju i MAPK/ERK signaliziranje u tkivu spojnice bolesnika s glaukomom
AuthorFilip Kelava
MentorMarin Ogorevc (mentor)
Committee memberSandra Kostić (predsjednik povjerenstva)
Committee memberIvana Gunjača (član povjerenstva)
Committee memberLjubo Znaor (član povjerenstva)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2024-09-03, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Ophthalmology
Abstract
Objectives: Our study aimed to investigate the proliferation and activation of MAPK/ERK signaling in the conjunctival cells of glaucoma patients who received PGF2 analogs in their treatment and those without PGF2 analog treatment. We hypothesized that the patient group treated with PGF2 analogs would show increased conjunctival cellular proliferation, MAPK/ERK activation, and a positive correlation between those two processes. Material and methods: Conjunctival samples were collected during deep sclerotomy procedures for the treatment of glaucoma and subsequently processed by standard preparation procedures for tissues before sectioning. Following immunofluorescence staining for Ki-67 and P-ERK1/2, the sections were examined by digital image analysis to quantify the emitted fluorescent signals. For the evaluation of statistically significant differences between the expression of the tested proteins in our sample groups, we used a two-way analysis of variance with Tukey’s post hoc test. Results: We found an increased proliferation rate, measured by Ki-67 expression, in the conjunctival cells of glaucoma patients treated with PGF2 analogs compared to patients without PGF2 analog treatment. The same trend was present when analyzing the activation of the MAPK/ERK pathway by measuring the expression of P-ERK1/2. Cellular proliferation and MAPK/ERK activation were markedly higher in the conjunctival epithelium compared to the stroma. There was no significant correlation between the proliferation rate and activation of MAPK/ERK signaling in the conjunctival cells of our samples. Conclusions: Our study has demonstrated a significant increase in conjunctival epithelial and stromal cell proliferation and activation of the MAPK/ERK pathway in glaucoma patients treated with PFG2 analogs. However, these two processes were not significantly correlated, indicating that MAPK/ERK activation may have other effects on the conjunctiva. Future studies on other PG-associated signaling pathways could elucidate the mechanism of increased proliferation in the conjunctiva and lead to potential novel targets for treating the side effects of PGF2 analog treatment.
Abstract (croatian)
Ciljevi: Cilj našeg istraživanja bio je odrediti stopu proliferacije i aktivacije mitogenima aktivirane protein kinaze / kinaze regulirane izvanstaničnim signalima (MAPK/ERK) signalnog puta u stanicama spojnice bolesnika s glaukomom koji su tijekom liječenja primali analoge prostaglandina F2 (PGF2), odnosni onih koji nisu liječeni analozima PGF2. Pretpostavili smo da će skupina bolesnika liječena analozima PGF2 pokazati povećanu proliferaciju i aktivaciju MAPK/ERK signalnog puta u stanicama spojnice te pozitivnu korelaciju između ta dva procesa. Materijali i metode: Uzorci spojnica prikupljeni su tijekom provođenja zahvata duboke sklerotomije za liječenje glaukoma i naknadno obrađeni standardnim postupcima za obradu tkiva prije izrade rezova. Nakon imunofluorescencijskog bojenja na Ki-67 i fosforilirani ERK1/2 (P-ERK1/2), provedena je digitalna analiza slika u svrhu kvantifikacije fluorescentnih signala. Za procjenu statistički značajnih razlika između izražaja ispitivanih proteina u našim uzorcima koristili smo dvosmjernu analizu varijance s Tukeyjevim post hoc testom. Rezultati: Pronašli smo povećanu stopu proliferacije, mjerenu izražajem Ki-67, u stanicama spojnice bolesnika s glaukomom liječenih analozima PGF2 u usporedbi s pacijentima bez liječenja analozima PGF2. Isti trend bio je prisutan kada se analizirala aktivacija MAPK/ERK signalnog puta mjerenjem izražaja P-ERK1/2. Stanična proliferacija i aktivacija MAPK/ERK signalnog puta bili su značajno veći u epitelu spojnice u usporedbi sa stromom. Nije bilo značajne povezanosti između stope proliferacije i aktivacije MAPK/ERK signalnog puta u stanicama spojnice naših uzoraka. Zaključci: Naše je istraživanje pokazalo značajno povećanje proliferacije epitelnih i stromalnih stanica spojnice i aktivaciju MAPK/ERK signalnog puta u bolesnika s glaukomom liječenih analozima PFG2. Međutim, nije postojala značajna povezanost ova dva procesa što ukazuje da aktivacija MAPK/ERK signalnog puta vjerojatno ima druge učinke na spojnicu. Daljnja istraživanja o drugim signalnim putevima povezanim s prostaglandinima mogla bi razjasniti mehanizam povećane proliferacije u spojnici te dovesti do potencijalnih novih meta za liječenje nuspojava analoga PGF2.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:171:693049
Study programmeTitle: Medical Studies in English
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-09-03
Terms of useLicence In copyright icon
Created on2024-08-30 07:11:18