| Abstract | Cilj istraživanja: Cilj istraživanja je ispitati potencijalno citotoksično djelovanje koloidnog srebra proizvedenog u zelenom čaju na humane stanice karcinoma glića maternice (HeLa i SiHa) i mokraćnog mjehura (T24 i TCCSUP). Materijali i metode: Citotoksičnost se ispitivala MTT metodom kojom se određuje postotak metabolički aktivnih stanica nakon tretiranja koloidnim srebrom proizvedenim u zelenom čaju. Uzorci su pripravljeni u različitim razrijeđenjima: 0,1%, 0,5%, 1%, 2,5%, 5%, 10%, 20%, 30%, 40% i 50%. Učinak je mjeren nakon 4, 24, 48 i 72 sata. Djelotvornost uzorakaa određena je spektrofotometrijski mjerenjem apsorbancije pri 570 nm. Rezultati: Rezultati koji predstavljaju odnos postotka metabolički aktivnih stanica i vremena inkubacije su prikazani grafički. Kod karcinoma glića maternice koloidno srebro je ostvarilo visok citotoksični učinak na Hela staničnu liniju. Najznačajniji pad metabolički aktivnih stanica dogodio se u najdužem intervalu (72 sata) uz primjenu 10%-tne otopine, te se primjenom većih koncentracija nije značajno smanjivao broj aktivnih stanica. IC50 se mogao odrediti za vremenske intervale od 24, 48 i 72 sata. Kod SiHa stanične linije IC50 mogao se odrediti samo za 48 i 72 sata, a citotoksični učinak je slabiji nego na HeLa liniju. Najizraženiji pad broja aktivnih stanica uočen je nakon 72 sata uz primjenu najkoncentriranije otopine (50%-tna). Kod karcinoma mokraćnog mjehura koloidno srebro je na T24 liniju ostvarilo sličan učinak kao i na HeLa liniju karcinoma grlića maternice. Najznačajniji pad metabolički aktivnih stanica dogodio se u najdužem intervalu (72 sata) uz primjenu 10%-tne otopine, te se primjenom većih koncentracija nije značajno smanjivao broj aktivnih stanica. IC50 se mogao odrediti nakon 48 i 72 sata. Najslabiji učinak koloidno srebro ostvarilo je na TCCSUP staničnu liniju. IC50 se nije mogao izmjeriti, a citotoksična aktivnost, iako mala, najizraženija je bila nakon 72 sata uz najvišu koncentraciju. Zaključci: In vitro izlaganje stanica karcinoma grlića maternice (HeLa i SiHa) i mokraćnog mjehura (T24 i TCCSUP) koloidnom srebru dovodi do smanjenja preživljenja tih stanica. Citotoksični učinak uglavnom je ovisan o vremenu inkubacije i koncentraciji. Najjači citotoksični učinak zabilježen je kod primjene 10%-tnih i koncentrirenijih otopina tijekom 72 sata na HeLa i T24 stanice. Potencijalno citotoksično djelovanje je dakle potvrđeno, a potrebna su daljnja in vivo ispitivanja kako bi se potvrdilo djelovanje i na životinjskim modelima. |
| Abstract (english) | Research Objective: The objective of the study was to examine the potential cytotoxic effects of colloidal silver produced in green tea on human cervical cancer cells (HeLa and SiHa) and bladder cancer cells (T24 and TCCSUP). Materials and Methods: Cytotoxicity was tested using the MTT method, which determines the percentage of metabolically active cells after treatment with colloidal silver produced in green tea. Samples were prepared in various dilutions: 0.1%, 0.5%, 1%, 2.5%, 5%, 10%, 20%, 30%, 40%, and 50%. The effect was observed after 4, 24, 48, and 72 hours. The efficacy of the extracts was determined spectrophotometrically by measuring absorbance at 570 nm. Results: The results are presented graphically in terms of the percentage of metabolically active cells and incubation time. In cervical cancer, colloidal silver showed a high cytotoxic effect on the HeLa cell line. The most significant drop in the number of metabolically active cells occurred in the longest interval (72 hours) with the application of a 10% solution, and using higher concentrations did not significantly reduce the number of active cells. IC50 was determined for the 24, 48, and 72-hour intervals. For the SiHa cell line, IC50 could only be determined at 48 and 72 hours, and the cytotoxic effect was not so good as on the HeLa line. The most visible drop in the number of active cells was observed after 72 hours with the application of the most concentrated solution (50%). In bladder cancer, colloidal silver showed a similar effect on the T24 line as on the HeLa cervical cancer line. The most significant drop in metabolically active cells occurred in the longest interval (72 hours) with the application of a 10% solution, and using higher concentrations did not significantly reduce the number of active cells. IC50 could be determined after 48 and 72 hours. The weakest effect of colloidal silver was observed on the TCCSUP cell line. IC50 could not be determined, and although cytotoxic activity was low, it was most visible after 72 hours with the highest concentration. Conclusions: In vitro exposure of cervical cancer cells (HeLa and SiHa) and bladder cancer cells (T24 and TCCSUP) to colloidal silver leads to a reduction in the survival of these cells. The cytotoxic effect is mostly dependent on the incubation time and concentration. The strongest cytotoxic effect was observed with the application of 10% and more concentrated solutions during 72 hours on HeLa and T24 cells. The potential cytotoxic effect is confirmed, but further in vivo studies are needed to confirm the effect in animal models. |
