prikaz prve stranice dokumenta Obrasci proliferacijske aktivnosti u zametnoj osnovi ljudskog zuba tijekom fetalnog razdoblja razvoja
  Download
PDF 2.8 MB
master's thesis
Obrasci proliferacijske aktivnosti u zametnoj osnovi ljudskog zuba tijekom fetalnog razdoblja razvoja
2017. urn:nbn:hr:171:788439
Stazić, Petra
University of Split
School of Medicine
Department of Anatomy, Histology and Embryology

Cite this document

Stazić, P. (2017). Obrasci proliferacijske aktivnosti u zametnoj osnovi ljudskog zuba tijekom fetalnog razdoblja razvoja (Master's thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:788439

Stazić, Petra. "Obrasci proliferacijske aktivnosti u zametnoj osnovi ljudskog zuba tijekom fetalnog razdoblja razvoja." Master's thesis, University of Split, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:171:788439

Stazić, Petra. "Obrasci proliferacijske aktivnosti u zametnoj osnovi ljudskog zuba tijekom fetalnog razdoblja razvoja." Master's thesis, University of Split, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:171:788439

Stazić, P. (2017). 'Obrasci proliferacijske aktivnosti u zametnoj osnovi ljudskog zuba tijekom fetalnog razdoblja razvoja', Master's thesis, University of Split, School of Medicine, accessed 06 April 2025, https://urn.nsk.hr/urn:nbn:hr:171:788439

Stazić P. Obrasci proliferacijske aktivnosti u zametnoj osnovi ljudskog zuba tijekom fetalnog razdoblja razvoja [Master's thesis]. Split: University of Split, School of Medicine; 2017 [cited 2025 April 06] Available at: https://urn.nsk.hr/urn:nbn:hr:171:788439

P. Stazić, "Obrasci proliferacijske aktivnosti u zametnoj osnovi ljudskog zuba tijekom fetalnog razdoblja razvoja", Master's thesis, University of Split, School of Medicine, Split, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:171:788439

Please login to the repository to save this object to your list.
Metadata
TitleObrasci proliferacijske aktivnosti u zametnoj osnovi ljudskog zuba tijekom fetalnog razdoblja razvoja
Title (english)The patterns of proliferation in a human tooth germ during the fetal developmental period
AuthorPetra Stazić
MentorDarko Kero (mentor)
GranterUniversity of Split
School of Medicine
(Department of Anatomy, Histology and Embryology)
Split
Defense date and country2017, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Dental Medicine
Morphology of the Dental Tissue System
Abstract
Cilj istraživanja: Cilj istraživanja je bio prikazati obrasce proliferacijske aktivnosti stanica u zametcima ljudskog zuba tijekom razvojnih stadija pupoljka, kape i zvona. Materijali i metode: U svrhu istraživanja korišteni su uzorci tkiva uzeti iz deset ljudskih fetusa starih 7, 8/9, 10 i 14 tjedana trudnoće dobivenih nakon spontanih pobačaja i ektopičnih trudnoća te preuzetih s Odjela patologije KBC-a Split. Imunohistokemijska analiza provedena je na fetalnom tkivu u području dijelova... More čeljusti koji su sadržavali zubne zametke. Rezovi su bojani primarnim monoklonskim protutijelima za Ki67 i p19. Preparati su fotografirani digitalnom kamerom postavljenom na fluorescencijski mikroskop, a dobivene slike su obrađene u AdobePhotoshop® CS6. Superponiranjem slika (merge) te obrađivanjem istih u ImageJ softveru procijenjivala se izražajna domena ispitivanih čimbenika. Rezultati: Proliferacijski aktivne stanice pozitivne na Ki67 uočene su u svim ispitivanim stadijima zubnog zametka. Zastupljene su podjednako u epitelnim i mezenhimnim dijelovima zubnog zametka, a početkom stadija zvona (histodiferencijacija) su bile najbrojnije u njegovim epitelnim dijelovima, odnosno unutarnjem caklinskom epitelu, stratum intermediumu i cervikalnim petljama. Izražajna domena p19 se uvelike preklapala s tkivima zubnog zametka u kojima su se nalazile stanice pozitivne na Ki67 u svim ispitivanim stadijima razvoja, ali je u stadiju zvona p19 bio izražen i u mezenhimnim dijelovima zubnog zametka (dentalna papila). Zaključak: S obzirom na postojeće znanje o osnovnoj funkciji p19 i rezultatima dobivenim u ovom istraživanju, može se zaključiti da p19 istovremeno sudjeluje u procesima proliferacije i diferencijacije u zametku zuba tijekom njegovog ranog razvoja te da je aktivnost p19 važno molekularno obilježje interakcija između epitelnih i mezenhminih dijelova zametka ljudskog zuba. Less
Abstract (english)
Objectives: The goal of research was to show patterns of cell proliferative activity in developing human tooth germ during bud, cap and bell stages. Material and Methods: For the purpose of the study, tissue samples were taken from ten human fetuses aged 7, 8/9, 10 and 14 weeks of gestation after spontaneous abortions and ectopic pregnancies from University Hospital Split Department of Pathology. Immunohistochemistry analysis was performed on fetal tissues from head area or parts of the... More jaw containing tooth germs. Tissue sections were stained with primary monoclonal antibodies against Ki67 and p19. Stained histological tissue sections were photographed by digital camera mounted on a fluorescence microscope, and the microphotographs were processed in AdobePhotoshop® CS6. Merged image doublets were then assembled and processed by using ImageJ software, to evaluate the expression domains of the examined factors. Results: Ki67 positive proliferating cells were observed in tissues of human tooth germ throughout the investigated period. Ki67 proliferating cells were distributed equally in the epithelial and mesenchymal parts of the tooth germ, whereas during the early bell stage (histodifferentiation) these cells were more localized in its epithelial parts, such as the inner enamel epithelium, stratum intermedium and cervical loops. Expression domains of p19 greatly overlapped with areas populated by Ki67 positive proliferating cells throughout the investigated period, but at the bell stage p19 was also expressed in the mesenchymal parts of the dental germ (the dental papilla). Conclusion: Based on the present knowledge about p19 cellular functions and results from this study, it might be suggested that p19 can simultaneously participate in proliferation and in differentiation processes during the early tooth development and that p19 activity is an important molecular feature of epithelial-mesenchymal interactions regulating basic cellular processes in developing human tooth germ. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:788439
Study programmeTitle: Dental Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica dentalne medicine (doktor/doktorica dentalne medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-11-24 13:41:51
accessibility

closeAccessibilityrefresh

If you wish to permanently save changes, click on Save, if not - your settings will be reset when you restart the browser.