prikaz prve stranice dokumenta Expression pattern of Syndecan-1 and HSP-70 in hip tissue of patients with osteoarthritis
  Download
PDF 2.73 MB
master's thesis
Expression pattern of Syndecan-1 and HSP-70 in hip tissue of patients with osteoarthritis
2019. urn:nbn:hr:171:793439
Kaufman, Julian
University of Split
School of Medicine
Hystology and embryology

Cite this document

Kaufman, J. (2019). Expression pattern of Syndecan-1 and HSP-70 in hip tissue of patients with osteoarthritis (Master's thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:793439

Kaufman, Julian. "Expression pattern of Syndecan-1 and HSP-70 in hip tissue of patients with osteoarthritis." Master's thesis, University of Split, School of Medicine, 2019. https://urn.nsk.hr/urn:nbn:hr:171:793439

Kaufman, Julian. "Expression pattern of Syndecan-1 and HSP-70 in hip tissue of patients with osteoarthritis." Master's thesis, University of Split, School of Medicine, 2019. https://urn.nsk.hr/urn:nbn:hr:171:793439

Kaufman, J. (2019). 'Expression pattern of Syndecan-1 and HSP-70 in hip tissue of patients with osteoarthritis', Master's thesis, University of Split, School of Medicine, accessed 05 December 2024, https://urn.nsk.hr/urn:nbn:hr:171:793439

Kaufman J. Expression pattern of Syndecan-1 and HSP-70 in hip tissue of patients with osteoarthritis [Master's thesis]. Split: University of Split, School of Medicine; 2019 [cited 2024 December 05] Available at: https://urn.nsk.hr/urn:nbn:hr:171:793439

J. Kaufman, "Expression pattern of Syndecan-1 and HSP-70 in hip tissue of patients with osteoarthritis", Master's thesis, University of Split, School of Medicine, Split, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:171:793439

Please login to the repository to save this object to your list.
Metadata
TitleExpression pattern of Syndecan-1 and HSP-70 in hip tissue of patients with osteoarthritis
Title (croatian)Ekspresija proteina sindekan-1 i HSP-70 u tkivu pacijenata s teškim osteoartritisom kuka
AuthorJulian Kaufman
MentorKatarina Vukojević (mentor)
Committee memberSandra Kostić (predsjednik povjerenstva)
Committee memberJoško Božić (član povjerenstva)
Committee memberFabijan Čukelj (član povjerenstva)
GranterUniversity of Split
School of Medicine
(Hystology and embryology)
Split
Defense date and country2019-07-12, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Cytology, Histology and Embryology
Abstract
Objectives: The purpose of this study is to investigate the expression of Syndecan-1 and HSP-70 in synovial, chondral and subchondral tissue in the hip of human patients with osteoarthritis according to their disease severity. Materials and methods: Total hip arthroplasty with tissue extraction from the weightbearing area of the pathologic femoral head was performed on all patients. Patients were divided into two groups according to their disease severity defined by their Kellgran Lawrence, Harris Hip and Oxford Hip Score: control (femoral neck fracture) and severe Osteoarthritis (OA). Chondral, subchondral and synovial tissues were analyzed with raised antibodies against Syndecan-1 and HSP-70. Results: Immunohistochemical staining with Syndecan-1 and HSP 70 antibodies revealed positive expression of both markers. It was established that the chondral, subchondral and synovial tissue in patients with severe OA contained abundant positive cells with a mean positive cell count of 120±16 and 142±23 for Syndecan-1 and 120±24 and 136±28 for HSP-70, respectively. On the contrary, patients without OA had only minimal positivity with a mean positive cell count of 30±4 and 34±16 for Syndecan-1 and 30±7 and 33±22 for HSP-70, respectively. Synovial tissue could not be identified enough for presentation. However, it followed the trend of chondral and subchondral tissue. It was established that there was a statistical significance of both Syndecan-1 and HSP-70 with p < 0.05 in cartilage and p < 0.001 in subchondral tissue between control and severe OA. Conclusions: Patients with severe osteoarthritis had increased staining of Syndecan-1 and HSP-70 in the cartilage and subchondral tissue of the hip compared to patients who underwent total hip replacement for a femoral neck fracture. Increased expression implies that Syndecan-1 and HSP-70 could play a significant role in the complex pathophysiology of osteoarthritis but further research is needed in order to make these findings applicable for clinical treatments.
Abstract (croatian)
Ciljevi: Svrha ovog istraživanja bila je usporediti razinu ekspresije proteina sindekan-1 i HSP-70 u sinoviji, hrskavici i subhondralnoj kosti pacijenata s teškim osteoartritisom (OA) kuka i pacijenata bez osteoartritisa. Materijali i metode: Sukladno težini pripadajuće bolesti, određenoj prema Kellgren-Lawrence, Harris Hip i Oxford Hip klasifikaciji, ispitanici su podijeljeni u dvije grupe: eksperimentalna grupa (teški OA) i kontrolna grupa (prijelom vrata femura). Svim ispitanicima učinjena je totalna artroplastika kuka s ekstrakcijom patološki oštećenog tkiva. Ekstrahirano tkivo sinovije, hrskavice i subhondralne kosti je imunohistokemijski analizirano pri čemu su korištena protutijela na sindekan-1 i HSP-70. Rezultati: Imunohistokemijska analiza s protutijelima na sindekan-1 i HSP-70 pokazala je pojačanu ekspresija obaju proteina u hrskavici (H) i subhondralnoj kosti (SHK) u eksperimentalnoj grupi u odnosu na kontrolnu. Zbog nedovoljno ekstrahiranog sinovijalnog tkiva, na sinoviji nije bilo moguće pouzdano usporediti grupe, no dojam je da bi rezultat bio sukladan onom u H i SHK. U pacijenata s teškim OA, u H i SHK, zabilježeno je obilje ''pozitivnih'' stanica sa srednjom vrijednošću od 120±16 i 142±23 za sindekan-1 te 120±24 i 136±28 za HSP-70. U pacijenata bez osteoartritisa zabilježena je blaga ekspresija ispitivanih proteina u H i SHK sa srednjom vrijednošću ''pozitivnih stanica'' od 30±4 i 34±16 za sindekan-1 te 30±7 i 33±22 za HSP-70. Utvrđena razlika u ekspresiji navedenih proteina statistički je značajna s razinom značajnosti p<0,05 za hrskavicu i p<0,001 za subhondralno tkivo. Zaključci: Ekspresija proteina sindekan-1 i HSP-70 u hrskavici i subhondralnoj kosti pacijenata s teškim osteoartritisom kuka je značajno veća u odnosu na pacijente bez osteoartritisa. Povećana ekspresija proteina sindekan-1 i HSP-70 sugerira moguću značajnu ulogu navedenih proteina u složenoj patofiziologiji osteoartritisa. Ipak, daljnja istraživanja su nužna da bi nalazi ovog istraživanja našli mjesto u kliničkoj promjeni.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:171:793439
Study programmeTitle: Medical Studies in English
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-07-16 07:35:59