Objectives: The expression and localization of Dab1 adaptor proteins and Notch2 receptors in the nephrons of yotari (Dab1 -/-), heterozygote (Dab1 +/-), and Wildtype (Dab1 +/+) mice was analyzed to further develop their suggested importance in mammal kidneys overall, but also their significance particularly in yotari mice nephrons. Materials and methods: yotari, heterozygote and wildtype mice were sacrificed on the 4th postnatal day. Paraffin embedded kidney tissue sections were analyzed by immunofluorescence using the antibodies, Notch2 and Dab1. Kidney structures were examined by fluorescence microscope. The percentage of positive cells between each group were compared and analyzed by a Kruskal-Wallis test. Results: In the DCT of all genotypes, a strong Dab1 expression signal was seen mostly to be localized in the cell membranes and was the structure with the highest percentage of immunoreactive cells for both Notch2 and Dab1. The highest percentage of positive cell expression for Notch2 stained kidneys was in the heterozygote genotype for all structures. In Dab1 immunofluorescence samples, the highest percentage of immunoreactive cells was observed in the DCT and glomeruli of heterozygotes and in the PCT of the wildtype genotype. Minimal positive cell expression in the yotari PCTs, and glomeruli of yotari and wildtype samples stained with Dab1 antibodies was found. There was minimal positive expression in the Notch2 stained wildtype glomeruli. Conclusions:The expression patterns of Dab1 and Notch2 in the nephron structures of the three genotypes of mice implicate not only the potential importance of Dab1 in DCT cellular signaling cascades for fluid and electrolyte homeostasis, but also suggests the possible signaling interaction of Dab1 and Notch2 in this structure. Overall, the yotari nephron showed significant DCT Dab1 expression, and variable Notch2 expression in all structures, with Notch2 expression in the glomeruli implicating glomerular injury as a possible cause of death in these knockout mice.