Objectives: The purpose of this research was to determine the level of AGEs in the general population and to evaluate their relationship with objective parameters of skin quality. Patients and methods: This cross-sectional observational study was conducted at the Department of Maxillofacial Surgery, Faculty of Medicine, University of Split. 162 respondents participated in the study. The examined sample was divided into two groups, younger (N=83) and older (N=79), while the limit was 40 years of age. The inclusion criteria were: age between 18 and 80 years; Fitzpatrick skin types I-III. Skin quality assessment was performed by the same experienced researcher using the MP6 skin quality assessment instrument. AGEs levels were measured by skin autofluorescence. All participants were subjected to measurements in a room where air conditions were carefully monitored. Results: It was found that the younger group has a statistically significantly higher level of skin hydration, friction and a significantly lower level of TEWL compared to the older group. No statistically significant difference was found in other objective skin parameters. Also, the younger group has a significantly lower level of AGEs compared to the older group. Correlation analysis between AGEs and objective skin parameters showed that there is a significant positive correlation with the level of melanin in both groups. Also, a significant negative correlation was found between AGEs and the level of skin hydration in both groups. Furthermore, a significant positive correlation was found between AGEs and TEWL levels in both groups. Conclusion: The younger group has a higher level of skin hydration, friction and a higher amount of AGEs, and lower level of TEWL compared to the older group. A positive correlation was found between AGEs and the level of melanin and TEWL in both groups. Also, a negative correlation was found between AGEs and the level of skin hydration in both groups.