Aim: The aim of this study was to examine the differences between male and female sex, i.e. sexual dimorphism in physical measures of the length and width of the crown and roots of the maxillary incisors and canines. Materials and methods: This study was based on measurements of total length, crown length, root length and crown width of maxillary incisors and canines on CBCT scans of live patients from southern Croatia. The subjects were divided into two groups according to gender. The measurements were made in the i-Dixel One Volume Viewer 2.8 computer program using software tools and were expressed in milliliters. The obtained data was included in a Microsoft Excel spreadsheet and statistical data processing was performed in the same program. The sexes were compared by one-way/single factor MANOVA and complemented by multiple post-hoc t-tests to detect the measures that contribute the most to the difference. Results: Among the 54 subjects of equal gender distribution, the age range was 27 to 64 years. Multiple Shapiro Wilk tests indicate a generally normal distribution of data. The MANOVA test found a statistically significant difference for all three types of measured teeth (P < 0.01 (P > 0.05)), and post-hoc analysis by t-tests indicated a statistically significant difference for all total length and length of roots, i.e. that these measures contribute the most to the determined sexual dimorphism. Conclusion: Upper frontal teeth are on average larger in males than in females, i.e. sexual dimorphism is present among the teeth of the population of southern Croatia. The most pronounced values are on the total length and the length of the roots.