Objectives: The aim of this study was to examine the cytotoxic effect of colloidal silver, produced using honey, on cervical cancer cells (HeLa and SiHa). Materials and methods: Cytotoxicity was assessed using the MTT assay, which evaluates the proportion of metabolically active cells after treatment with colloidal silver derived from honey. Samples were prepared in different concentrations: 0.1%, 0.5%, 1%, 2.5%, 5%, 10%, 20%, 30%, 40%, 50%. The effects of treatment were evaluated after 4, 24, 48, and 72 hours. The effectiveness of the samples was determined spectrophotometrically by measuring absorbance at a wavelength of 570 nm. Results: The results are presented graphically, showing the relationship between the percentage of metabolically active cells and the incubation time and the sample concentration. The study confirmed that colloidal silver produced using honey exhibits significant cytotoxic effects on cervical cancer cells, especially the HeLa cell line, while the effect on SiHa cells was somewhat weaker. In HeLa cells, the most pronounced cytotoxic effect was observed after 72 hours of treatment with a 20% colloidal silver solution, where further increases in concentration did not significantly reduce the number of metabolically active cells. On the other hand, in SiHa cells, the greatest decrease in the number of metabolically active cells was observed after 72 hours of treatment with a 50% solution. IC50 values were determined for the time intervals of 24, 48, and 72 hours. Conclusions: In vitro exposure of cervical cancer cells (HeLa and SiHa) to colloidal silver resulted in a reduction in their viability. Cytotoxicity depended on the concentration of colloidal silver and the incubation time, with the strongest effects observed after 72 hours of treatment and at higher concentrations. The potential cytotoxic effect was confirmed, but further in vitro and in vivo studies are needed to verify its effectiveness.