OBJECTIVE: The main aim of this research was to define most common isolates from hemo culture an their sensibility to different antibiotics, using the retrospective analysis in Unit of intensive care of the Department of anesthesiology, reanimatology and intensive care UHC Split in 2015. Based on facts regarding the most common causes and the most successful antibiotic treatment in last year, patients in next year would be able to get more successful initial therapy. MATERIALS AND METHODS: Retrospective, cohort research included all patients treated in ICU of UHC Split, 104 of them whose hemocultures were positive in 2015. RESULTS: Results didnt show expected higher percentage of gram negative infective agents comparing to gram positive. As well as percentage of fungi as infective agents was very low. The difference between gram negative and gram positive agents is rather high. But the expectation is that difference will decrease. So in the future gram negative agents will have higher percent. In this research there were 63,1% gram positive agents 35,4% gram negative agents and 1,3% of fungi. CONCLUSION: Problems facing ICUs in other countries are increased gram negative sepsis and inefficacy of antibiotics in ICU of UHC Split are not that much expressed. Gram positive agents prevail, and the most serious ones show sufficinet sensitivity to antibiotics. Increased percentage of fungi is also not recorded, which is good because of the severitry of infections, high mortality, and also increased cost of tretment.