OBJECTIVES: The main aim of this study was to determine pathohistological characteristics of liver tissue samples in University Hospital of Split in the period from January 01st, 2015 till December 31st, 2018. Secondary aim was to determine by which diagnostic procedure liver tissue samples were obtained and incidence of diagnosis depending on the gender of the patients. MATERIALS AND METHODS: Data were collected by reviewing the database of the Department of Pathology, Clinical Institute of Pathology, Forensic Medicine and Citology of Split, and 448 pathohistological findings were noted with the code of liver organ. All listed pathohistological findings were included in the study. Data on the age and sex of patients, the year of sampling, the type of material, the refferal diagnosis, the pathohistological diagnosis and primary site of the metastases were obtained. RESULTS: 448 liver tissue samples were received in the Department of Pathology, Clinical Institute of Pathology, Forensic Medicine and Citology at University Hospital of Split. 245 (54.7%) patients were male. The median age for men was 63 (8-88), and for women 60 (1-87). By using ultrasound guided biopsy, 369 (82.41%) liver tissue samples were obtained and the remaining 79 (17.6%) were obtained by surgical procedure. The most common refferal diagnosis, under which liver samples were admitted for pathohistological analysis, was liver metastasis. 121 (27%) samples were adressed as liver metastasis. 148 (33%) liver tissue samples did not have stated refferal diagnosis. During pathohistological analysis, in 135 (30.1%) patients the diagnosis of liver metastasis was made and it was the most common pathohistological diagnosis. The most frequent liver metastasis originated from colorectal carcinoma or the origin was stated as the unknown, which was reported in 36 (26.7%) cases. CONCLUSION: Liver biopsy is the gold standard for diagnosing liver metastases. Considering incidence and anatomical connection, metastases of colorectal carcinoma are the most common secondary liver neoplasms. Without the consideration of the patients age nor gender, ultrasound guided liver biopsy was more often in use for sampling liver tissue than surgical procedure. To make a good pathohistological diagnosis, it is important to establish a good communication between pathologist and clinician.