Objectives: The aim of this study was to compare cognitive and psychomotor performance between men and women and to compare daytime sleepiness assessed with the Epworth sleepiness scale (ESS) and Stanford sleepiness scale (SSS), subjective sleep quality assessed with Pittsburgh sleep quality index (PSQI) and sleep habits between men and women. Furthermore, we investigated the association between cognitive and psychomotor performance on three tests of the computer based system Complex Reactionmeter Drenovac (CRD-series), and daytime sleepiness assessed and subjective sleep. Patients and methods: A total of 168 medical students from the University of Split School of Medicine were included in the research: 49 men with a median age of 20, ranging from 19 to 25, and 119 women with a median age of 21, ranging from 19 to 23. All students filled in the SSS, ESS, PSQI and sleep habits questionnaire. Afterwards the students performed three tests on the CRD-series battery (CRD11, CRD311, CRD411). Results: In the PSQI there was a statistically significant higher score in women (5.68±2.53) compared to men (4.80±2.14, P=0.023). The SSS score was also significantly higher in women in contrast to men (2.82±1.22 vs. 2.23±0.98, respectively, P=0.002). Women also stated to sleep shorter (6h35min ± 1h 8min) than men (6h 58min ± 58 min, P=0.024). Chronic tiredness was significantly more frequent among women than men (P=0.007). On the CRD11 test men had statistically significant shorter MinT and TTST (TTST 94.46±14.84 s and MinT 1.61±0.23 s) than women (TTST 105.82±17.78 s and MinT 1.78±0.28 s, P<0.001 for both comparisons). In the CRD411 men were also significantly faster than women (TTST 26.94±4.76 s vs. 31.22±7.12 s for TTST and 0.30±0.07s vs. 0.43±0.08 s for MinT, respectively, P<0.001 for both comparisons). On the CRD311 test women needed significantly more time to complete the whole test than men (TTST was 28.18±2.46 s in women and 27.43±2.09 s in men, P=0.047). No significant correlation was found between the performance on CRD series test and sleepiness, assessed with the ESS and SSS, sleep quality assessed with PSQI and subjective sleep duration. Conclusion: Our results showed that men had shorter reaction times than women on three tests (CRD11, CRD311 and CRD411) of the CRD-series, while women had shorter sleep duration and worse sleep quality assessed with PSQI than men and experienced more daytime sleepiness assessed with SSS but there was no difference in daytime sleepiness assessed with ESS between men and women. We found no correlation between daytime sleepiness and performance on the tests of the CRD-series and between sleep quality and performance on the tests of the CRD-series.