Objectives: The objective of this research was to determine a subjective experience of comfort and pain of patients having absorbable and non-absorbable suture materials in their oral cavity, as well as to determine objective factors of tissue healing, wound cleanliness and suture holding strength. In addition, the objective was to determine whether there was a difference in tissue healing, wound cleanliness, and suture holding strength with respect to the existence of systemic disease, taking medicines and cigarette smoking. Materials and methods: A total of 40 respondents participated in the research, who were divided into two groups based on the material with which the wound was sutured. There were 18 respondents in the group whose wound was sutured with non-absorbable material (Corolene®) and 22 respondents in the group whose wound was sutured with absorbable material (PGA Rapid). The research was conducted by means of an anonymous questionnaire composed of 15 questions and a clinical examination. Seven days after wound suturing procedure the respondents assessed a subjective feeling of comfort and pain having the stitches in their oral cavity; whereas the dental medicine practitioner assessed the wound cleanliness, tissue healing and suture holding strength during the follow-up appointment. Results: The results confirmed the previous findings that absorbable materials are more comfortable for patients, as opposed to non-absorbable materials, which have proved to be less comfortable, but produce a very low tissue reaction and do not support the development of inflammation and infection. No statistically significant difference regarding tissue healing, wound cleanliness and suture holding strength was found in respondents who were healthy compared to those suffering from a systemic disease, among smokers and non-smokers as well as among respondents who were on chronic drug therapy compared to those who were not. Conclusions: Based on the obtained results, it can be concluded that the absorbable material is very comfortable for patients. Although uncomfortable, the non-absorbable material produces a very low tissue reaction and does not support the onset of inflammation and infection. Further research is needed on a larger sample size to assess the impact of systemic disease, taking medicines and cigarette smoking on tissue healing, wound cleanliness and suture holding strength.