Aim of the study: The aim of this study was to evaluate the knowledge and experiences of parents of preschool children about the oral health of their children. Materials and methods: 297 parents of children of kindergarten age fromRepublic of Croatia participated in this research. The research was conducted using a questionnaire created and shared via a Google form. The questionnaire contained questions of parents' knowledge and experiences about the period of teething, oral hygiene in their children and breastfeeding. We also examined their familiarity with preventive procedures and habits of consuming sweetened beverages in their children and about the first visit to the dentist and the application of local anesthesia in children. Results: Only 24% of the respondents answered that the child should be taken to the dentist by the age of one. 36% of respondents think that their child has at least one tooth that they think is carious. 55.6% of parents of preschool children believe that breastfeeding has no influence on the development of teeth, and even 75.1% of them believe that breastfeeding cannot cause tooth decay. 39% of the surveyed parents answered that it is necessary to breastfeed as long as possible. A large number of parents, as many as 67% of them, believe that a five-year-old child is independent enough to maintain his own oral hygiene. 60.6% of respondents are not familiar with the concept of fissure sealing. 24.6% of parents still believe that local anesthesia is contraindicated in children. Conclusion:The knowledge of parents of pre-school children in Dalmatia about maintaining children's oral hygiene is unsatisfactory. Parents are not familiar with the methods of prevention in dental medicine or the time of the first visit to the dentist, which confirms the need for better education of parents. Parents' knowledge about the connection between breastfeeding and dental health and development of teeth is also insufficient.