Objectives: The aim of this research is to determine the knowledge and attitudes of the students of the University of Split, School of Medicine about the pharmacological treatment of anxiety and to assess the current level of anxiety among the examined students. Respondents and methods: The respondents included in this research are students of the University of Split, School of Medicine in the academic year 2021/2022. Knowledge and attitudes about the pharmacological treatment of anxiety and assessment of students' current anxiety were examined using an anonymous questionnaire that contained 46 questions divided into 4 parts, which include: sociodemographic characteristics, examination of attitudes, examination of knowledge and assessment of students' current anxiety. Results: A total of 589 students of the University of Split, School of Medicine participated in the study. Students showed positive attitudes towards people suffering from anxiety. Although older students showed better knowledge about the disease itself and about the drugs used for treatment (p<0.001) than younger students, still around 25,0% of them did not recognize a common side effect of antidepressants (decreased libido). In this research, 38.5% of students expressed that they felt a certain degree of anxiety, and the highest percentage were medical students in English (60.8%). Conclusion: Senior students of University of Split, School of Medicine showed that they have positive attitudes and more knowledge about the pharmacological treatment of anxiety than students of lower years. However, students generally showed that they do not have enough knowledge to recognize side effects. One third of the students suffer from anxiety, among them mostly medical students in English language. This research shows that education contributes to the reduction of prejudices and that it would be good to provide students with professional psychological support to help them cope with problems students with professional psychological support to help them cope with problems.