Objectives: After completing their studies and entering the professional world, newly graduated dentists, despite comprehensive theoretical and practical university education, feel a lack of confidence in their clinical practice and ability to make decisions. This study aimed to examine the level of satisfaction of sixth-year dental students and graduate dentists with knowledge and skills acquired during their university studies. Materials and methods: This cross-sectional study was conducted through an online questionnaire in which student responses were measured on a 5-point Likert satisfaction scale. A total of 533 respondents participated, 51 (28.3%) were dental students, and 382 (71.7%) were dentists. The data were analyzed using the Kruskal-Wallis test and the Mann-Whitney test. The level of significance was set at 0.05. Results: By comparing the satisfaction assessment between dentists and dental students on learned basic knowledge and skills during their studies, students showed a higher level of satisfaction for the categories "Emergency medical conditions (syncope, anaphylaxis, toxic reaction, etc.)" (P=0.005) and "Ergonomic Principles of Work" (P=0.004), while dentists showed a higher level of satisfaction for the categories "Selection and application of local anaesthetics" (P=0.005) and "Selection and use of local antibiotics" (P=0.026). Furthermore, in the type of technical skills from different branches of dental medicine, dentists were most satisfied with "Oral Surgery" (4.26±0.85), while students with "Oral Medicine" (4.29±0.89). By comparing the satisfaction assessment between dentists and dental students with personal skills learned during their studies, dental practitioners showed a lower level of satisfaction for all the categories studied (P≤0.05). Conclusion: Sixth-year dental students showed higher overall satisfaction than dental practitioners. The most significant satisfaction was expressed in learned technical skills, and the most considerable dissatisfaction was recorded in the category of "Immeasurable skills" with an emphasis on "Management" and "Career Management", which is why changes in the curricular program of dental medicine are recommended.