The aim of this study: The aim of this research is to determine how training at different intensities (below or above basal metabolism) affects monocyte subpopulations in a sample of recreational athletes. Methods: Blood samples of all participants were taken for analysis 30 minutes before and after training. The sample was treated with Fc receptor blocking reagent and incubated for 20 minutes in the dark at 25°C with 4 μL of anti-human CD14s PerCP-Cy5.5 conjugated antibodies and 5 μL of phycoerythrin-conjugated antibodies reactive to human CD16. After the lysis of red blood cells with lysis solution, the cells were analyzed on a flow cytometer. Samples of unstained cells were also measured and processed as negative controls. Data collected by cytometer were analyzed using FlowLogic software. Results: As a result of the research, we obtained an information that physical activity has an impact on the activation of anti-inflammatory and inflammatory monocyte groups. By increasing the intensity of physical activity, an increase in the number of monocyte groups was observed. The results also tell that the time of blood sampling and the number of subjects have an influence on results. Conclusion: Training of different intensity in recreational athletes (below or above basal metabolism) affects the increase in the number of inflammatory and anti-inflammatory monocytes, but for a better understanding of intensity influence, it is necessary to take into account the time of blood sampling and a larger sample.