Endothelial plasma membrane lipid microdomains, so called lipid rafts/caveolae, are rich in neutral glycosphingolipid, globotriaosylceramide, Gb3Cer or CD77. Several plasma membrane Ca2+ channels and pumps are located in lipid rafts/caveolae. Increased Ca2+ influx could cause the development of an endothelial proinflammatory phenotype. Therefore, the aim of this study was to estimate effects of hypercalcemia in rats by determination of CD77 expression on CD34+ endothelial cells in heart, kidney and vena cava. In addition, potential proinflammatory calcium effect was estimated by CD11b and CD15s expression on leukocytes. To achieve hypercalcemia, Sprague-Dawley male rats were drinking CaCl2 solution with a concentration of 1.5% elemental calcium during 14 days. CD77 expression on CD34+ endothelial cells in cell suspensions of heart, kidney and vena cava, as well as leukocyte expression of CD11b and CD15s in hypercalcemic and control rats were determined by flow cytometry. Ionized calcium concentration in plasma was 1.37  0.01 mM in hypercalcemic vs. 1.19  0.03 mM in control rats. Hypercalcemic group showed statistically significantly decreased proportion of endothelial CD34+CD77- cells in the kidney and vena cava in parallel with increase of percentage of leucocytes expressiong proinflammatory markers CD11b and CD15s. In conclusion, it is tempting to speculate that plasma membranes of glycosphingolipid CD77- endothelial cells are poorer in caveolae lipid microdomains and therefore weaker in controlling of Ca2+ influx. The percentage of CD11b+CD15s+ leukocytes could be a measure of proinflammatory effects of mild hypercalcemia.