Background/aims: Testicular torsion is a condition that requires immediate diagnosis and treatment in order to preserve the vitality of the testicles and possible fertility. Many studies about the effect of various drugs and substances that have a protective effect on the testicle in T/D injury has been published but none become entrenched in everyday practice. The aim of this study was to investigate the effect of nifedipine on the unilateral testicular T/D injury in rats. Methods: Twenty-four adult male Sprague-Dawley rats, aged 9 weeks (adult rats) were randomly divided into 3 groups, each containing 8 rats. Rats in the control group underwent a sham operation of the left testis. In the T/D group, the left testis was twisted at 720° for 3 hours. After 3 hours of reperfusion, at the end of the experiment, the testes were removed. Rats in the treatment group received the same surgical procedure as the T/D group, but nifedipine was administered intraperitoneally 30 minutes before the time of detorsion. After an additional 3 hours of reperfusion bilateral orchiectomy was performed. Removed testes were analyzed for oxidative stress (activity of the enzyme glutathione peroxidase, superoxide dismutase and malondialdehyde in testicular tissue). Histological analysis by Johnsen’s criteria was performed and apoptosis was measured. Results: In the group of rats with unilateral T/D injury, treated with nifedipine, ipsilateral testis showed less damage compared to the group that did not receive nifedipine (lower oxidative stress and apoptotic index). There were no significant differences in Johnsen’s criteria after a unilateral I/R injury (3 hours torsion and 3 hours detorsion). Also no significant differences in contra lateral testis after unilateral I/R injury were recorded except in oxidative stress. Conclusion: Nifedipine has protective effect on ipsilateral testicle after unilateral acute T/D injury of the rat’s testicle.