OBJECTIVES: The aim of this study was to assess clinical features, laboratory parameters and treatment of the patients with HLA-B35 positive spondyoarthritides in Split-Dalmatia County. METHODS: The study included 64 patients with HLA-B35 positive spondyloarthritis. The data were collected from archives of the Department of Rheumatology and Clinical Immunology, University Hospital Split for the period from January 2005 to December 2015. All patients were evaluated by the ASAS classification criteria for the axial or peripheral spondyloarthritis. RESULTS: A female predominance with a female/male ratio of 4,82/1 was documented. 68,7% of patients exhibited axial manifestations of the disease, while 90,6% had peripheral manifestations. 14,1% of the 64 patients showed eye involvement and 20,3% skin involvement. Not a single patient had inflammatory bowel disease. 15,6% of patients were HLA-B27 positive. The difference in laboratory values of C-reactive protein and erythrocyte sedimentation rate was not statistically significant between the patients who exhibited axial and peripheral involvement. NSAIDs were used by 81,2% of patients included in the study, followed by DMARDs 75%, physical therapy 48,4%, systemic glucocorticoids 29,7%, topical NSAIDs 25% and glucocorticoid injections 4,7%. CONCLUSION: Our data suggest that there is a significant proportion of patients having axial and peripheral manifestations among HLA-B35 positive patients with spondyloarthritis. On the other hand only small number of patients showed extraarticular involvement. Also, the frequency of concomitant HLA-B27 positivity was low. The most common treatment were NSAIDs.