Aim of the study: Aim of this study is to determine the frequency of Candida spp. causing the candidemia in University Hospital Centre Split during the period of 2011. - 2015. and also to determine if there is a trend of an increase of the non-albicans isolates, and antifungal sensitivity of a species causing the candidemia. Patients and methods: In this retrospective study data has been used from the archive of Department for Microbiology and Parasitology, University Hospital Centre Split, about Candida isolates and their antifungal resistance pattern during the period 2011.-2015. Overall, 4763 isolates were analyzed of which 149 isolates were from the hemoculture. For data analysis a method of an increase and a decrease in a trend (the exponential trend method) was used. Difference in a proportion has been tested with the one way Z test. Representativeness of a method was tested with the ANOVA method. Statistical analysis has been performed with the STATISTICA 12. Results: In the studied period C. albicans is still the main cause of a candidemia (62%). When considering the hemoculture, we found that there is a decrease of C. albicans (38%) and an increase in the non-albicans (62%) species (p=0.001766). The most frequently isolated non-albicans species was C. parapsilosis (24%). C. albicans showed good sensitivity to the antifungal agents tested. C. parapsilosis isolates showed highest resistance to fluconazole (53%). Furthermore, C. parapsilosis isolates showed 40% resistance rate to voriconazole and C. glabrata strains showed 27% resistance rate to fluconazole. Conclusion: When the overall Candida isolates are analyzed it is established that C. albicans is still the most frequent isolate in University Hospital Centre Split. When considering the isolates from the hemocultures it is established that there is a trend of an increase in the non-albicans species from which the most frequent is C. parapsilosis. Non-albicans species, especially C. parapsilosis showed higher resistance to the antifungal agents, primarily fluconazole, so we recomend usage of the echinocandins when the non-albicans candidemia is suspected. If a cause of the candidemia is identified as C. albicans, echinocandins should be replaced with the azoles. Local epidemiological surveillance of a causative agents of a candidemia is important and also a surveillance of a sensitivity of the Candida spp. to the antifungal agents.