Objectives: Quality of life is substantially impaired in patients with CD by being a chronic, remitting and unpredictable entity adversely affecting day to day functioning. The aim of this study was to determine health-related quality of life in patients with Crohn’s disease. Subjects and methods: A total of 30 patients diagnosed with CD performed the Inflammatory Bowel Disease Questionnaire (IBDQ-32) questionnaire consisting of 32 items in order to assess their quality of life and specific patterns potentially characteristic for Split-Dalmatia County. Results: No statistically significant differences were found in patients with active and not active disease related to quality of life. Perception of quality of life for these patients was positively correlated with overall score (r=0.506; P=0.004) and interestingly, t a positive correlation was found between their quality of life and gastrointestinal symptoms but it was not statistically significant. Systemic symptoms (r=0.629; P<0.001), emotional involvement (r=0.588; P<0.001), together with social impact (r=0.458; P<0.011) domains were all positively correlated with self-perception quality of life. Additionally, no significant correlations between disease duration and any of the studied IBDQ domains was found. The overall score was 162.5 and 189.5 for active and not active disease respectively. The highest score was observed in social domain while the lowest score was observed in systemic symptoms domain. Conclusion: This study confirmed the correlation between overall score, systemic symptoms, social and emotional domains related to HRQOL of patients with Crohn’s disease supporting the reliability of IBDQ-32 to assess HRQOL in patients suffering from this condition.